Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22950 | 5' | -51.5 | NC_005137.2 | + | 60644 | 0.7 | 0.858484 |
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Target: 5'- -gUGUUUGAUuugGCCAccGACCGACAGCGg -3' miRNA: 3'- cgACGAGCUG---UGGU--UUGGUUGUCGUa -5' |
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| 22950 | 5' | -51.5 | NC_005137.2 | + | 74122 | 0.7 | 0.857668 |
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Target: 5'- aGUUGUaCGugACCAccgcguuaauucuGACCGGCAGCGa -3' miRNA: 3'- -CGACGaGCugUGGU-------------UUGGUUGUCGUa -5' |
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| 22950 | 5' | -51.5 | NC_005137.2 | + | 13137 | 0.71 | 0.841745 |
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Target: 5'- uCUGCUCGAUAC--GACC-GCAGCGUu -3' miRNA: 3'- cGACGAGCUGUGguUUGGuUGUCGUA- -5' |
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| 22950 | 5' | -51.5 | NC_005137.2 | + | 69968 | 0.72 | 0.776925 |
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Target: 5'- aGUUGCaCGACACCG-ACC-ACAGCGg -3' miRNA: 3'- -CGACGaGCUGUGGUuUGGuUGUCGUa -5' |
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| 22950 | 5' | -51.5 | NC_005137.2 | + | 24549 | 0.73 | 0.746765 |
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Target: 5'- gGCgGCcUGACGCCGcguCCAACAGCAa -3' miRNA: 3'- -CGaCGaGCUGUGGUuu-GGUUGUCGUa -5' |
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| 22950 | 5' | -51.5 | NC_005137.2 | + | 81716 | 1.07 | 0.006841 |
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Target: 5'- uGCUGCUCGACACCAAACCAACAGCAUc -3' miRNA: 3'- -CGACGAGCUGUGGUUUGGUUGUCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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