Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22952 | 3' | -56.5 | NC_005137.2 | + | 57427 | 0.66 | 0.867298 |
Target: 5'- uUGGCCAGCaaCGCGUuGGUGGcCGaaaUGCa -3' miRNA: 3'- -ACCGGUUG--GCGUA-CCGCCuGUa--ACGc -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 55516 | 0.66 | 0.859637 |
Target: 5'- uUGGCCA---GCGUGGCcaagGGGCAcgUGCa -3' miRNA: 3'- -ACCGGUuggCGUACCG----CCUGUa-ACGc -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 48784 | 0.66 | 0.859637 |
Target: 5'- cGGCgCcGCacaGCGUGuGCGGcacuACAUUGCGc -3' miRNA: 3'- aCCG-GuUGg--CGUAC-CGCC----UGUAACGC- -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 35673 | 0.66 | 0.846945 |
Target: 5'- cGGCCGGCCGgaacgauugcguagaCGUGGUGGuGCuggGCa -3' miRNA: 3'- aCCGGUUGGC---------------GUACCGCC-UGuaaCGc -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 93277 | 0.66 | 0.846945 |
Target: 5'- cGGCCAGCCGCcgguuUuugcuaccuugaaacGGCGuGACGUcaagcuguUGCGu -3' miRNA: 3'- aCCGGUUGGCGu----A---------------CCGC-CUGUA--------ACGC- -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 88028 | 0.66 | 0.826955 |
Target: 5'- cGGCC-ACCGCcgaGGCGGGCcaccacGUGg -3' miRNA: 3'- aCCGGuUGGCGua-CCGCCUGuaa---CGC- -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 112581 | 0.66 | 0.824381 |
Target: 5'- cGGCCGACUccaacgccguacuuGCAcaaUGGCGcGCcgUGCGc -3' miRNA: 3'- aCCGGUUGG--------------CGU---ACCGCcUGuaACGC- -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 17461 | 0.67 | 0.818311 |
Target: 5'- cGGCCGuAUCGCAggguguggggcUGGUGGugG-UGCa -3' miRNA: 3'- aCCGGU-UGGCGU-----------ACCGCCugUaACGc -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 116369 | 0.67 | 0.818311 |
Target: 5'- gGGCUugagaAACCGCugaucGGUGGACAcuucuUUGCa -3' miRNA: 3'- aCCGG-----UUGGCGua---CCGCCUGU-----AACGc -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 96405 | 0.67 | 0.817437 |
Target: 5'- cGcGCCGACCGCuacccGcGCGGcgacgcgcccgccGCGUUGCGu -3' miRNA: 3'- aC-CGGUUGGCGua---C-CGCC-------------UGUAACGC- -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 73952 | 0.67 | 0.800511 |
Target: 5'- uUGGCCAACCuGCAgcaggccgUGGCGaACGagGCu -3' miRNA: 3'- -ACCGGUUGG-CGU--------ACCGCcUGUaaCGc -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 63000 | 0.67 | 0.800511 |
Target: 5'- cGGCgGACC-CAUGGUGcGGCGccgGCGu -3' miRNA: 3'- aCCGgUUGGcGUACCGC-CUGUaa-CGC- -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 41627 | 0.67 | 0.791372 |
Target: 5'- aGGUCAAcguCCGCu--GCGGACAgcgcgUGCGc -3' miRNA: 3'- aCCGGUU---GGCGuacCGCCUGUa----ACGC- -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 34588 | 0.67 | 0.775502 |
Target: 5'- cGGCCGACgcguuguaucaaaGCAccgaauUGGCGGACAauuugauuggcgguUUGCGc -3' miRNA: 3'- aCCGGUUGg------------CGU------ACCGCCUGU--------------AACGC- -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 117727 | 0.67 | 0.772661 |
Target: 5'- -cGUCAGCCGCAccGGCGGcGCAcuUUGCc -3' miRNA: 3'- acCGGUUGGCGUa-CCGCC-UGU--AACGc -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 106857 | 0.67 | 0.772661 |
Target: 5'- cGGCguuugCGGCUGCggugGUGGCGGGCAcgGCc -3' miRNA: 3'- aCCG-----GUUGGCG----UACCGCCUGUaaCGc -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 79888 | 0.68 | 0.763108 |
Target: 5'- cGGCCAuugcgUCGCAauuugcGGCGGGCAgcgGCa -3' miRNA: 3'- aCCGGUu----GGCGUa-----CCGCCUGUaa-CGc -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 27574 | 0.68 | 0.740699 |
Target: 5'- gGGCCAgcuguuacagcGCUGCAgcggccgcuacuuuUGGcCGGACGgcgUGCGu -3' miRNA: 3'- aCCGGU-----------UGGCGU--------------ACC-GCCUGUa--ACGC- -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 33767 | 0.68 | 0.723799 |
Target: 5'- aUGGCCAuugUGCGcGGCGG-CGUUGUGc -3' miRNA: 3'- -ACCGGUug-GCGUaCCGCCuGUAACGC- -5' |
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22952 | 3' | -56.5 | NC_005137.2 | + | 106568 | 0.69 | 0.672912 |
Target: 5'- cGGUCAAUa-CAUGGUGGACAUUuacgGCGc -3' miRNA: 3'- aCCGGUUGgcGUACCGCCUGUAA----CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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