Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22952 | 5' | -57.8 | NC_005137.2 | + | 70004 | 0.66 | 0.780441 |
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Target: 5'- aAUUGCaauguaucACCCGCCGgcaAUCCCaCGGCa -3' miRNA: 3'- -UAGCGguu-----UGGGCGGU---UAGGG-GUCGa -5' |
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| 22952 | 5' | -57.8 | NC_005137.2 | + | 95218 | 0.66 | 0.780441 |
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Target: 5'- uGUCGUgAAggGCuaGCCAAUUgCCAGCUg -3' miRNA: 3'- -UAGCGgUU--UGggCGGUUAGgGGUCGA- -5' |
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| 22952 | 5' | -57.8 | NC_005137.2 | + | 74938 | 0.66 | 0.759474 |
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Target: 5'- cUCGCCAAugCUGCCGucaacacAUCCUugucgucaccaaaCAGCa -3' miRNA: 3'- uAGCGGUUugGGCGGU-------UAGGG-------------GUCGa -5' |
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| 22952 | 5' | -57.8 | NC_005137.2 | + | 114412 | 0.66 | 0.741898 |
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Target: 5'- uUCcCCGugcGACCCGCCAAg-UCCGGCUa -3' miRNA: 3'- uAGcGGU---UUGGGCGGUUagGGGUCGA- -5' |
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| 22952 | 5' | -57.8 | NC_005137.2 | + | 3844 | 0.67 | 0.730996 |
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Target: 5'- -cCGCUAAugUUGCCGAUCUagaaacuCCAGCg -3' miRNA: 3'- uaGCGGUUugGGCGGUUAGG-------GGUCGa -5' |
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| 22952 | 5' | -57.8 | NC_005137.2 | + | 106693 | 0.67 | 0.721997 |
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Target: 5'- --gGCCGuGCCCGCCAccaCCgCAGCc -3' miRNA: 3'- uagCGGUuUGGGCGGUua-GGgGUCGa -5' |
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| 22952 | 5' | -57.8 | NC_005137.2 | + | 122382 | 0.67 | 0.691575 |
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Target: 5'- -aCGCCcacGCCCGCCg--CCUUAGCg -3' miRNA: 3'- uaGCGGuu-UGGGCGGuuaGGGGUCGa -5' |
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| 22952 | 5' | -57.8 | NC_005137.2 | + | 41499 | 0.75 | 0.302211 |
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Target: 5'- cGUCGCCAaugAACCCGCgCGugugCUCCAGCa -3' miRNA: 3'- -UAGCGGU---UUGGGCG-GUua--GGGGUCGa -5' |
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| 22952 | 5' | -57.8 | NC_005137.2 | + | 87378 | 1.05 | 0.002512 |
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Target: 5'- gAUCGCCAAACCCGCCAAUCCCCAGCUu -3' miRNA: 3'- -UAGCGGUUUGGGCGGUUAGGGGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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