miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22954 5' -50 NC_005137.2 + 89195 1.11 0.00657
Target:  5'- uAGGGAAACGCAAAGAAACGGUCGCUCa -3'
miRNA:   3'- -UCCCUUUGCGUUUCUUUGCCAGCGAG- -5'
22954 5' -50 NC_005137.2 + 69168 0.66 0.994536
Target:  5'- -cGGAuGCGCGccAGAAugGcGUCGCUg -3'
miRNA:   3'- ucCCUuUGCGUu-UCUUugC-CAGCGAg -5'
22954 5' -50 NC_005137.2 + 123371 0.68 0.979408
Target:  5'- uGGGu-AC-CGGA-AAACGGUCGCUCa -3'
miRNA:   3'- uCCCuuUGcGUUUcUUUGCCAGCGAG- -5'
22954 5' -50 NC_005137.2 + 52602 0.69 0.953542
Target:  5'- cGGcGGAGGCGCuu-GAAACGGaagCGCg- -3'
miRNA:   3'- -UC-CCUUUGCGuuuCUUUGCCa--GCGag -5'
22954 5' -50 NC_005137.2 + 16377 0.7 0.949222
Target:  5'- -cGGAAacgugGCGCAAAGAAGCuaugCGCUCg -3'
miRNA:   3'- ucCCUU-----UGCGUUUCUUUGcca-GCGAG- -5'
22954 5' -50 NC_005137.2 + 112476 0.7 0.949222
Target:  5'- uGGGAAugGUGGAGA-ACGGgaCGCUg -3'
miRNA:   3'- uCCCUUugCGUUUCUuUGCCa-GCGAg -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.