Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22955 | 3' | -60.6 | NC_005137.2 | + | 33775 | 0.71 | 0.389463 |
Target: 5'- -uGUGCGCGgCGGCGuuGUgcugUGCCa- -3' miRNA: 3'- gcCACGCGCgGUCGCggCAa---ACGGcu -5' |
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22955 | 3' | -60.6 | NC_005137.2 | + | 110722 | 0.72 | 0.327549 |
Target: 5'- aCGGUGUGCGCgCagAGCGCUuGUgcGCCGGg -3' miRNA: 3'- -GCCACGCGCG-G--UCGCGG-CAaaCGGCU- -5' |
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22955 | 3' | -60.6 | NC_005137.2 | + | 43782 | 0.76 | 0.205609 |
Target: 5'- ---aGCGCGCCAGCGg-GUUUGCCGAc -3' miRNA: 3'- gccaCGCGCGGUCGCggCAAACGGCU- -5' |
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22955 | 3' | -60.6 | NC_005137.2 | + | 35707 | 0.76 | 0.181988 |
Target: 5'- gCGGUGCGUGUCuugacaAGCGCCGUgUUGCCc- -3' miRNA: 3'- -GCCACGCGCGG------UCGCGGCA-AACGGcu -5' |
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22955 | 3' | -60.6 | NC_005137.2 | + | 93470 | 1.09 | 0.000939 |
Target: 5'- aCGGUGCGCGCCAGCGCCGUUUGCCGAa -3' miRNA: 3'- -GCCACGCGCGGUCGCGGCAAACGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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