Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22956 | 5' | -57.7 | NC_005137.2 | + | 59564 | 0.67 | 0.775298 |
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Target: 5'- gCGCUGGCCGUGUucagccuGGUGUaCGACAa- -3' miRNA: 3'- -GCGGCCGGCGCAu------CUACGcGUUGUcc -5' |
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| 22956 | 5' | -57.7 | NC_005137.2 | + | 3623 | 0.67 | 0.793548 |
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Target: 5'- uGUCGGCCGCGcugAGGUuGCGUuuCAa- -3' miRNA: 3'- gCGGCCGGCGCa--UCUA-CGCGuuGUcc -5' |
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| 22956 | 5' | -57.7 | NC_005137.2 | + | 130116 | 0.66 | 0.828262 |
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Target: 5'- aGUCGGaaacgcaCGCGUGGGUuGCGgCGauucuGCAGGa -3' miRNA: 3'- gCGGCCg------GCGCAUCUA-CGC-GU-----UGUCC- -5' |
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| 22956 | 5' | -57.7 | NC_005137.2 | + | 61516 | 0.66 | 0.836522 |
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Target: 5'- uCGCCGGCCGCccauuugacGcAGA-GCGCGugcCAGa -3' miRNA: 3'- -GCGGCCGGCG---------CaUCUaCGCGUu--GUCc -5' |
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| 22956 | 5' | -57.7 | NC_005137.2 | + | 59957 | 0.66 | 0.836522 |
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Target: 5'- aGCUGGCuCGCG-AGAUuuacgcGCGCAACc-- -3' miRNA: 3'- gCGGCCG-GCGCaUCUA------CGCGUUGucc -5' |
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| 22956 | 5' | -57.7 | NC_005137.2 | + | 30155 | 0.66 | 0.836522 |
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Target: 5'- uCGUCGGCCuu----GUGCGCAACAGa -3' miRNA: 3'- -GCGGCCGGcgcaucUACGCGUUGUCc -5' |
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| 22956 | 5' | -57.7 | NC_005137.2 | + | 123495 | 0.66 | 0.844598 |
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Target: 5'- uCGUCGGgCGCGguug-GCGCAGCGu- -3' miRNA: 3'- -GCGGCCgGCGCaucuaCGCGUUGUcc -5' |
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| 22956 | 5' | -57.7 | NC_005137.2 | + | 101156 | 0.73 | 0.451535 |
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Target: 5'- uGUaCGGCCGCGguUGGAcagGCGUGGCAGGc -3' miRNA: 3'- gCG-GCCGGCGC--AUCUa--CGCGUUGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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