Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22958 | 3' | -44.9 | NC_005137.2 | + | 34242 | 0.66 | 0.999995 |
Target: 5'- aUCGGUGCacaaacacgaaugGCCGCAAGGcaGAU--ACGAc -3' miRNA: 3'- -AGCUAUG-------------UGGCGUUCUa-CUAuuUGCU- -5' |
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22958 | 3' | -44.9 | NC_005137.2 | + | 73163 | 0.66 | 0.999987 |
Target: 5'- --aAUAC-CCGCAAGAauccaUGGUGGugGAc -3' miRNA: 3'- agcUAUGuGGCGUUCU-----ACUAUUugCU- -5' |
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22958 | 3' | -44.9 | NC_005137.2 | + | 48907 | 0.67 | 0.999943 |
Target: 5'- aCGAUcGCGCCGCcuuguucggcgacaaGAGcgGcAUGGACGAg -3' miRNA: 3'- aGCUA-UGUGGCG---------------UUCuaC-UAUUUGCU- -5' |
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22958 | 3' | -44.9 | NC_005137.2 | + | 52088 | 0.68 | 0.999887 |
Target: 5'- aCGGUACAUCGUAAGuGUGcgGAcaguaaucGCGAa -3' miRNA: 3'- aGCUAUGUGGCGUUC-UACuaUU--------UGCU- -5' |
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22958 | 3' | -44.9 | NC_005137.2 | + | 6979 | 0.68 | 0.999851 |
Target: 5'- aCGAUGCugCGCAcacgGAcGAGCGGu -3' miRNA: 3'- aGCUAUGugGCGUucuaCUaUUUGCU- -5' |
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22958 | 3' | -44.9 | NC_005137.2 | + | 59057 | 0.69 | 0.99975 |
Target: 5'- gCGA---ACCGCAAGAUuGUGAGCGu -3' miRNA: 3'- aGCUaugUGGCGUUCUAcUAUUUGCu -5' |
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22958 | 3' | -44.9 | NC_005137.2 | + | 48412 | 0.7 | 0.99901 |
Target: 5'- gCGAUgACGCCG-AGGAUG-UGGGCGAc -3' miRNA: 3'- aGCUA-UGUGGCgUUCUACuAUUUGCU- -5' |
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22958 | 3' | -44.9 | NC_005137.2 | + | 107823 | 0.7 | 0.998783 |
Target: 5'- gUGGU--GCCGCAAGAguUGAUAAACa- -3' miRNA: 3'- aGCUAugUGGCGUUCU--ACUAUUUGcu -5' |
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22958 | 3' | -44.9 | NC_005137.2 | + | 79903 | 0.71 | 0.998195 |
Target: 5'- aUCGAUgagGCGCCGCAAG-UGGUcAAUGc -3' miRNA: 3'- -AGCUA---UGUGGCGUUCuACUAuUUGCu -5' |
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22958 | 3' | -44.9 | NC_005137.2 | + | 109602 | 0.71 | 0.997384 |
Target: 5'- aCGAaACGCCGCGuuGGGUGAccGAUGGg -3' miRNA: 3'- aGCUaUGUGGCGU--UCUACUauUUGCU- -5' |
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22958 | 3' | -44.9 | NC_005137.2 | + | 106496 | 1.12 | 0.023793 |
Target: 5'- gUCGAUACACCGCAAGAUGAUAAACGAu -3' miRNA: 3'- -AGCUAUGUGGCGUUCUACUAUUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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