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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22958 | 5' | -43.6 | NC_005137.2 | + | 33968 | 0.67 | 0.999993 |
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Target: 5'- -gUCGUUgccgCCGCCauuaagcUCCGAguuGCAACg -3' miRNA: 3'- caAGCAAa---GGUGGa------AGGUUau-UGUUG- -5' |
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| 22958 | 5' | -43.6 | NC_005137.2 | + | 77295 | 0.68 | 0.999971 |
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Target: 5'- -aUCGUUgacgCUgguugacgACCUUCCAGcAACAACu -3' miRNA: 3'- caAGCAAa---GG--------UGGAAGGUUaUUGUUG- -5' |
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| 22958 | 5' | -43.6 | NC_005137.2 | + | 94104 | 0.76 | 0.967585 |
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Target: 5'- --aCGUUUCCACgUUUCAGUuuguGCAACa -3' miRNA: 3'- caaGCAAAGGUGgAAGGUUAu---UGUUG- -5' |
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| 22958 | 5' | -43.6 | NC_005137.2 | + | 106542 | 1.13 | 0.025631 |
|
Target: 5'- uGUUCGUUUCCACCUUCCAAUAACAACg -3' miRNA: 3'- -CAAGCAAAGGUGGAAGGUUAUUGUUG- -5' |
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| 22958 | 5' | -43.6 | NC_005137.2 | + | 27293 | 0.79 | 0.899189 |
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Target: 5'- cUUCGUUgcUCCACCUgaaCGGUGGCGGCg -3' miRNA: 3'- cAAGCAA--AGGUGGAag-GUUAUUGUUG- -5' |
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| 22958 | 5' | -43.6 | NC_005137.2 | + | 12357 | 0.74 | 0.991914 |
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Target: 5'- --gCGUcgUCCACCUUCCA-UAGCAc- -3' miRNA: 3'- caaGCAa-AGGUGGAAGGUuAUUGUug -5' |
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| 22958 | 5' | -43.6 | NC_005137.2 | + | 42186 | 0.66 | 1 |
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Target: 5'- -gUCGcgUCCGCCga-CAggGACGGCg -3' miRNA: 3'- caAGCaaAGGUGGaagGUuaUUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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