Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22959 | 3' | -48.3 | NC_005137.2 | + | 128780 | 0.68 | 0.993186 |
Target: 5'- cCGUGGGCg--UGCuAACCuACAaacuauuaaccgGCGGCa -3' miRNA: 3'- -GUACUCGaaaACG-UUGGcUGU------------UGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 37831 | 0.68 | 0.992981 |
Target: 5'- -cUGAGCgcuagcaucgUGCuGACCGGCGACGcGUu -3' miRNA: 3'- guACUCGaaa-------ACG-UUGGCUGUUGC-CG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 47406 | 0.68 | 0.991998 |
Target: 5'- gGUGAucaguuuGCgUUUGUacGACCG-CAGCGGCg -3' miRNA: 3'- gUACU-------CGaAAACG--UUGGCuGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 75709 | 0.66 | 0.998972 |
Target: 5'- aAUGAGUUUgUGUAcaaacgagacgccGCCGGucaagcuguuuguCAACGGCa -3' miRNA: 3'- gUACUCGAAaACGU-------------UGGCU-------------GUUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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