Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22961 | 3' | -57.1 | NC_005137.2 | + | 108067 | 1.06 | 0.002811 |
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Target: 5'- cGUGGAACACGCGACGCGGGGCCAAAAc -3' miRNA: 3'- -CACCUUGUGCGCUGCGCCCCGGUUUU- -5' |
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| 22961 | 3' | -57.1 | NC_005137.2 | + | 12872 | 0.74 | 0.371115 |
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Target: 5'- cUGGAuCACG-GGCGCGGGGCgAAAc -3' miRNA: 3'- cACCUuGUGCgCUGCGCCCCGgUUUu -5' |
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| 22961 | 3' | -57.1 | NC_005137.2 | + | 97988 | 0.71 | 0.496617 |
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Target: 5'- --cGGGCGCGCGGCGUGGGugcgcccGCCAAGu -3' miRNA: 3'- cacCUUGUGCGCUGCGCCC-------CGGUUUu -5' |
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| 22961 | 3' | -57.1 | NC_005137.2 | + | 125049 | 0.71 | 0.507386 |
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Target: 5'- -cGGAGcCGgGCGGCGCGGcGCCGGAu -3' miRNA: 3'- caCCUU-GUgCGCUGCGCCcCGGUUUu -5' |
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| 22961 | 3' | -57.1 | NC_005137.2 | + | 50040 | 0.7 | 0.598521 |
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Target: 5'- aUGGAACACGUGuuGCGccGCCAAGAc -3' miRNA: 3'- cACCUUGUGCGCugCGCccCGGUUUU- -5' |
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| 22961 | 3' | -57.1 | NC_005137.2 | + | 36029 | 0.69 | 0.619227 |
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Target: 5'- aUGuGAACACG-GGCGCGGGcGUUAAAAg -3' miRNA: 3'- cAC-CUUGUGCgCUGCGCCC-CGGUUUU- -5' |
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| 22961 | 3' | -57.1 | NC_005137.2 | + | 96357 | 0.69 | 0.671023 |
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Target: 5'- cGUGGugcgugcuUACGCcGCGaCGGGGCCAAc- -3' miRNA: 3'- -CACCuu------GUGCGcUGC-GCCCCGGUUuu -5' |
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| 22961 | 3' | -57.1 | NC_005137.2 | + | 31802 | 0.67 | 0.780441 |
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Target: 5'- uUGGcGCA-GCGAUGCGGcGGCCc--- -3' miRNA: 3'- cACCuUGUgCGCUGCGCC-CCGGuuuu -5' |
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| 22961 | 3' | -57.1 | NC_005137.2 | + | 31353 | 0.66 | 0.789759 |
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Target: 5'- cUGGuACAaggGCGACGCG-GGCCGGu- -3' miRNA: 3'- cACCuUGUg--CGCUGCGCcCCGGUUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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