Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22961 | 5' | -55.5 | NC_005137.2 | + | 108101 | 1.05 | 0.004342 |
Target: 5'- gAGUUGUGGUACGCGUUGCGCGCCGAUc -3' miRNA: 3'- -UCAACACCAUGCGCAACGCGCGGCUA- -5' |
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22961 | 5' | -55.5 | NC_005137.2 | + | 45270 | 0.73 | 0.496114 |
Target: 5'- aAGUUGUGcG-ACGCGUcGCGCGCCc-- -3' miRNA: 3'- -UCAACAC-CaUGCGCAaCGCGCGGcua -5' |
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22961 | 5' | -55.5 | NC_005137.2 | + | 43741 | 0.73 | 0.515917 |
Target: 5'- ---cGUGGUAgCGCGUaagcUGCGCGCCa-- -3' miRNA: 3'- ucaaCACCAU-GCGCA----ACGCGCGGcua -5' |
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22961 | 5' | -55.5 | NC_005137.2 | + | 61349 | 0.73 | 0.515917 |
Target: 5'- --gUGUGGUGCGUGUacauUGUGCGgCGAg -3' miRNA: 3'- ucaACACCAUGCGCA----ACGCGCgGCUa -5' |
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22961 | 5' | -55.5 | NC_005137.2 | + | 94285 | 0.69 | 0.73229 |
Target: 5'- --gUGUGGcGCGCGaguuuaauuguUUGCGCGCCa-- -3' miRNA: 3'- ucaACACCaUGCGC-----------AACGCGCGGcua -5' |
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22961 | 5' | -55.5 | NC_005137.2 | + | 75961 | 0.69 | 0.752151 |
Target: 5'- cGUUGUcGUgACGCGgcGCGCGuuGGUg -3' miRNA: 3'- uCAACAcCA-UGCGCaaCGCGCggCUA- -5' |
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22961 | 5' | -55.5 | NC_005137.2 | + | 119419 | 0.67 | 0.817685 |
Target: 5'- cGUUGUGGUGCGCacgGCaGCGuuGc- -3' miRNA: 3'- uCAACACCAUGCGcaaCG-CGCggCua -5' |
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22961 | 5' | -55.5 | NC_005137.2 | + | 18233 | 0.67 | 0.859415 |
Target: 5'- uGUUGcUGuGUGCGCGUcguucguacUGCGgCGCCGc- -3' miRNA: 3'- uCAAC-AC-CAUGCGCA---------ACGC-GCGGCua -5' |
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22961 | 5' | -55.5 | NC_005137.2 | + | 20541 | 0.66 | 0.866383 |
Target: 5'- uGGUUGUGGUGCGaGUUucacaaagcgcacGCGUaCCGAg -3' miRNA: 3'- -UCAACACCAUGCgCAA-------------CGCGcGGCUa -5' |
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22961 | 5' | -55.5 | NC_005137.2 | + | 99349 | 0.66 | 0.881942 |
Target: 5'- ---cGUGGUACGCGUcggaacaaUGCacgGCGCCc-- -3' miRNA: 3'- ucaaCACCAUGCGCA--------ACG---CGCGGcua -5' |
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22961 | 5' | -55.5 | NC_005137.2 | + | 37592 | 0.66 | 0.895812 |
Target: 5'- ---cGUGGUGCGCGgcgGCaaucaCGCUGAUc -3' miRNA: 3'- ucaaCACCAUGCGCaa-CGc----GCGGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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