Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22962 | 5' | -44.2 | NC_005137.2 | + | 9819 | 0.66 | 0.999995 |
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Target: 5'- gCGCuGCAAguugaaauguggucuGAAGACGGUGUugAAGAAUAu -3' miRNA: 3'- -GCG-CGUU---------------CUUCUGCUACA--UUUUUGUu -5' |
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| 22962 | 5' | -44.2 | NC_005137.2 | + | 24063 | 0.66 | 0.999997 |
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Target: 5'- aUGCGCugguuGGAGGGCGAUGa-------- -3' miRNA: 3'- -GCGCGu----UCUUCUGCUACauuuuuguu -5' |
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| 22962 | 5' | -44.2 | NC_005137.2 | + | 27782 | 0.7 | 0.99923 |
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Target: 5'- gCGUGCAGGcAAGACGuaaccgauuuUGUAAAAAUAu -3' miRNA: 3'- -GCGCGUUC-UUCUGCu---------ACAUUUUUGUu -5' |
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| 22962 | 5' | -44.2 | NC_005137.2 | + | 41162 | 0.7 | 0.999386 |
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Target: 5'- gGCGC-AGAcGGCGGUGUGGuAGGCGu -3' miRNA: 3'- gCGCGuUCUuCUGCUACAUU-UUUGUu -5' |
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| 22962 | 5' | -44.2 | NC_005137.2 | + | 46158 | 0.77 | 0.941263 |
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Target: 5'- gGCGUAaaAGGAGGCGGUGUugAGAAACGu -3' miRNA: 3'- gCGCGU--UCUUCUGCUACA--UUUUUGUu -5' |
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| 22962 | 5' | -44.2 | NC_005137.2 | + | 58358 | 0.69 | 0.999768 |
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Target: 5'- uCGUGCAAGAAGACGcacuUGcAAAAguACAu -3' miRNA: 3'- -GCGCGUUCUUCUGCu---ACaUUUU--UGUu -5' |
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| 22962 | 5' | -44.2 | NC_005137.2 | + | 70228 | 0.68 | 0.999943 |
|
Target: 5'- gGCGUcgguGAAGGCGucgGUGAAGGCGu -3' miRNA: 3'- gCGCGuu--CUUCUGCua-CAUUUUUGUu -5' |
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| 22962 | 5' | -44.2 | NC_005137.2 | + | 70288 | 0.68 | 0.999943 |
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Target: 5'- gGCGUcgguGAAGGCGucgGUGAAGGCGu -3' miRNA: 3'- gCGCGuu--CUUCUGCua-CAUUUUUGUu -5' |
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| 22962 | 5' | -44.2 | NC_005137.2 | + | 108198 | 1.08 | 0.037753 |
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Target: 5'- aCGCGCAAGAAGACGAUGUAAAAACAAc -3' miRNA: 3'- -GCGCGUUCUUCUGCUACAUUUUUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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