Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22965 | 3' | -51.7 | NC_005137.2 | + | 85689 | 0.66 | 0.98059 |
Target: 5'- aAUUUgCAUgAGCGAAGAGAaacgcaugGUGGCCg -3' miRNA: 3'- cUAAGgGUGgUCGCUUUUCU--------CGUCGG- -5' |
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22965 | 3' | -51.7 | NC_005137.2 | + | 43793 | 0.66 | 0.978261 |
Target: 5'- gGGUUUgCCGacuuUCAGCGAcggcacGAGCAGCCg -3' miRNA: 3'- -CUAAG-GGU----GGUCGCUuuu---CUCGUCGG- -5' |
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22965 | 3' | -51.7 | NC_005137.2 | + | 92813 | 0.66 | 0.978261 |
Target: 5'- ---aCCCACguGCGAAAAaugaGGCGGUa -3' miRNA: 3'- cuaaGGGUGguCGCUUUUc---UCGUCGg -5' |
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22965 | 3' | -51.7 | NC_005137.2 | + | 99595 | 0.66 | 0.978261 |
Target: 5'- ---gCCCGCCGGUGugccuGGUGGCCu -3' miRNA: 3'- cuaaGGGUGGUCGCuuuucUCGUCGG- -5' |
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22965 | 3' | -51.7 | NC_005137.2 | + | 57850 | 0.66 | 0.978017 |
Target: 5'- cAUUUUUugCAGCGcgGAGAGCAuguaccgcacacgGCCa -3' miRNA: 3'- cUAAGGGugGUCGCuuUUCUCGU-------------CGG- -5' |
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22965 | 3' | -51.7 | NC_005137.2 | + | 130579 | 0.67 | 0.966856 |
Target: 5'- cGUUgCCACCAGCGAAucaacCAGCg -3' miRNA: 3'- cUAAgGGUGGUCGCUUuucucGUCGg -5' |
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22965 | 3' | -51.7 | NC_005137.2 | + | 27396 | 0.68 | 0.927203 |
Target: 5'- ---gCCguCCGGCcAAAAGuAGCGGCCg -3' miRNA: 3'- cuaaGGguGGUCGcUUUUC-UCGUCGG- -5' |
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22965 | 3' | -51.7 | NC_005137.2 | + | 125008 | 0.69 | 0.902846 |
Target: 5'- gGAUgCCgCACCGGCGAucGGuuGCGGCg -3' miRNA: 3'- -CUAaGG-GUGGUCGCUuuUCu-CGUCGg -5' |
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22965 | 3' | -51.7 | NC_005137.2 | + | 48906 | 0.69 | 0.896109 |
Target: 5'- cGAUcgCgCCGCCuuguucGGCGAcAAGAGCGGCa -3' miRNA: 3'- -CUAa-G-GGUGG------UCGCUuUUCUCGUCGg -5' |
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22965 | 3' | -51.7 | NC_005137.2 | + | 47290 | 0.7 | 0.889119 |
Target: 5'- ----aCCGCUAGCGAAuGGAGUaauuguggguuGGCCg -3' miRNA: 3'- cuaagGGUGGUCGCUUuUCUCG-----------UCGG- -5' |
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22965 | 3' | -51.7 | NC_005137.2 | + | 112576 | 1.13 | 0.002919 |
Target: 5'- aGAUUCCCACCAGCGAAAAGAGCAGCCg -3' miRNA: 3'- -CUAAGGGUGGUCGCUUUUCUCGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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