Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22965 | 5' | -57.8 | NC_005137.2 | + | 62958 | 0.66 | 0.775467 |
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Target: 5'- -cAGaUUCUgGCCCAAAGCGuuCGGCGa -3' miRNA: 3'- auUC-GAGAgCGGGUUUCGCc-GCCGUc -5' |
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| 22965 | 5' | -57.8 | NC_005137.2 | + | 96201 | 0.66 | 0.765924 |
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Target: 5'- --cGUUCgUCGCUaaguuGGGCGGCGGUGGc -3' miRNA: 3'- auuCGAG-AGCGGgu---UUCGCCGCCGUC- -5' |
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| 22965 | 5' | -57.8 | NC_005137.2 | + | 57721 | 0.66 | 0.746479 |
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Target: 5'- cUGGGCUCgacgCGuUCUAAAcGCGGCGGgGGc -3' miRNA: 3'- -AUUCGAGa---GC-GGGUUU-CGCCGCCgUC- -5' |
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| 22965 | 5' | -57.8 | NC_005137.2 | + | 34824 | 0.66 | 0.736596 |
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Target: 5'- -cGGCgCUUGCUgGu-GCGGUGGCGGg -3' miRNA: 3'- auUCGaGAGCGGgUuuCGCCGCCGUC- -5' |
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| 22965 | 5' | -57.8 | NC_005137.2 | + | 42527 | 0.67 | 0.71656 |
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Target: 5'- --cGCUCgccCGCCCGAaucGGCaacaGGUGGCAa -3' miRNA: 3'- auuCGAGa--GCGGGUU---UCG----CCGCCGUc -5' |
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| 22965 | 5' | -57.8 | NC_005137.2 | + | 9671 | 0.67 | 0.68597 |
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Target: 5'- --cGCUgUUCGUCaacgugacAAGCGGCGGCAGc -3' miRNA: 3'- auuCGA-GAGCGGgu------UUCGCCGCCGUC- -5' |
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| 22965 | 5' | -57.8 | NC_005137.2 | + | 70122 | 0.67 | 0.674637 |
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Target: 5'- aAAGUacaugggCUCGCCCAAcgguucgggacuaGGUGGCGGUg- -3' miRNA: 3'- aUUCGa------GAGCGGGUU-------------UCGCCGCCGuc -5' |
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| 22965 | 5' | -57.8 | NC_005137.2 | + | 103805 | 0.68 | 0.665331 |
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Target: 5'- -uAGCagUUGCUgGuguGCGGCGGCGGg -3' miRNA: 3'- auUCGagAGCGGgUuu-CGCCGCCGUC- -5' |
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| 22965 | 5' | -57.8 | NC_005137.2 | + | 33268 | 0.68 | 0.634187 |
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Target: 5'- --uGCUCaUGUCCAucaauuGCGGCGGCGu -3' miRNA: 3'- auuCGAGaGCGGGUuu----CGCCGCCGUc -5' |
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| 22965 | 5' | -57.8 | NC_005137.2 | + | 112610 | 1.06 | 0.001948 |
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Target: 5'- aUAAGCUCUCGCCCAAAGCGGCGGCAGu -3' miRNA: 3'- -AUUCGAGAGCGGGUUUCGCCGCCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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