Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22967 | 3' | -51.6 | NC_005137.2 | + | 47893 | 0.66 | 0.982103 |
Target: 5'- aCGUACCGCAGCuGAAcgUGUCuuCGCc -3' miRNA: 3'- aGCGUGGUGUCGuUUUagACAG--GCGu -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 123252 | 0.66 | 0.979904 |
Target: 5'- -aGCGCUGCAGCAAAAUCcuUCaaGCAu -3' miRNA: 3'- agCGUGGUGUCGUUUUAGacAGg-CGU- -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 63633 | 0.66 | 0.979904 |
Target: 5'- aCGcCGCCGCGGCGAcg---GUCgCGCAu -3' miRNA: 3'- aGC-GUGGUGUCGUUuuagaCAG-GCGU- -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 118065 | 0.66 | 0.979904 |
Target: 5'- cCGCGCCGCAGCGA---UUGUaauauaaGCAa -3' miRNA: 3'- aGCGUGGUGUCGUUuuaGACAgg-----CGU- -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 99620 | 0.66 | 0.977511 |
Target: 5'- gCGCGCCGCgccaAGCAAcuccuuUCUgGUCaCGCAu -3' miRNA: 3'- aGCGUGGUG----UCGUUuu----AGA-CAG-GCGU- -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 89094 | 0.67 | 0.962331 |
Target: 5'- uUCGUuuuuCCACAaac-AGUCUGUCCGCu -3' miRNA: 3'- -AGCGu---GGUGUcguuUUAGACAGGCGu -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 120274 | 0.67 | 0.958601 |
Target: 5'- cUCGCGCCAUggAGCccGAGGUgcagCUGUCCGa- -3' miRNA: 3'- -AGCGUGGUG--UCG--UUUUA----GACAGGCgu -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 122578 | 0.67 | 0.958601 |
Target: 5'- gCGCGCUGCAGCAAAAc----CCGCGu -3' miRNA: 3'- aGCGUGGUGUCGUUUUagacaGGCGU- -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 90853 | 0.67 | 0.953383 |
Target: 5'- aCGCACCAcCAGCGGAuaauaaaagucggcGUCgcGUCCGg- -3' miRNA: 3'- aGCGUGGU-GUCGUUU--------------UAGa-CAGGCgu -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 115444 | 0.68 | 0.950398 |
Target: 5'- aUCGC-CCGCAGC----UC-GUCCGCu -3' miRNA: 3'- -AGCGuGGUGUCGuuuuAGaCAGGCGu -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 18380 | 0.68 | 0.950398 |
Target: 5'- aCGCGCaCACAGCAAcaccgccUUGUCCGg- -3' miRNA: 3'- aGCGUG-GUGUCGUUuua----GACAGGCgu -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 119866 | 0.68 | 0.94908 |
Target: 5'- gCGCugCGCGGCGGGAUgcguacgugcuaagCUGUgUGCGc -3' miRNA: 3'- aGCGugGUGUCGUUUUA--------------GACAgGCGU- -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 2160 | 0.68 | 0.930913 |
Target: 5'- cUGCGCCGCAGCGugcacgagCUGUgCaCGCGg -3' miRNA: 3'- aGCGUGGUGUCGUuuua----GACA-G-GCGU- -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 48201 | 0.68 | 0.92595 |
Target: 5'- -gGCACgCGCGGCAAAuggaacaaugacccuUCcGUCCGCGa -3' miRNA: 3'- agCGUG-GUGUCGUUUu--------------AGaCAGGCGU- -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 31834 | 0.69 | 0.913542 |
Target: 5'- cCGCACaaccaCGCGGCGGuGUC-GUCCGCu -3' miRNA: 3'- aGCGUG-----GUGUCGUUuUAGaCAGGCGu -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 2390 | 0.7 | 0.871831 |
Target: 5'- aUCGCGCCAaaugGGUAA----UGUCCGCAa -3' miRNA: 3'- -AGCGUGGUg---UCGUUuuagACAGGCGU- -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 64076 | 0.71 | 0.847712 |
Target: 5'- gCGCACCGCGuGCGAA--CcGUCCGCc -3' miRNA: 3'- aGCGUGGUGU-CGUUUuaGaCAGGCGu -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 13147 | 0.71 | 0.839231 |
Target: 5'- aCG-ACCGCAGCGuuGUUUGUuugCCGCAg -3' miRNA: 3'- aGCgUGGUGUCGUuuUAGACA---GGCGU- -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 13890 | 0.71 | 0.830542 |
Target: 5'- gCGCugCACGGCGucuucUUUGUCgGCAa -3' miRNA: 3'- aGCGugGUGUCGUuuu--AGACAGgCGU- -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 100221 | 0.71 | 0.821654 |
Target: 5'- gCGCGCCGCGGCAcAAAgcaugCUGaacgcgCCGCGc -3' miRNA: 3'- aGCGUGGUGUCGU-UUUa----GACa-----GGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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