Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22967 | 3' | -51.6 | NC_005137.2 | + | 79846 | 0.74 | 0.691403 |
Target: 5'- gUGCGCCaaguaacGCAGCGGGGUC-GUCCGCc -3' miRNA: 3'- aGCGUGG-------UGUCGUUUUAGaCAGGCGu -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 62218 | 0.75 | 0.638999 |
Target: 5'- uUUGCGCUGCcGCGA--UCUGUCCGCu -3' miRNA: 3'- -AGCGUGGUGuCGUUuuAGACAGGCGu -5' |
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22967 | 3' | -51.6 | NC_005137.2 | + | 119075 | 1.1 | 0.005129 |
Target: 5'- cUCGCACCACAGCAAAAUCUGUCCGCAa -3' miRNA: 3'- -AGCGUGGUGUCGUUUUAGACAGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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