Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22967 | 5' | -54.4 | NC_005137.2 | + | 63001 | 0.74 | 0.518457 |
Target: 5'- --gGCGGACCC--AUGG-UGCGGCGCc -3' miRNA: 3'- uugCGCCUGGGcaUACUaACGUCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 108581 | 0.7 | 0.726098 |
Target: 5'- --gGCGGcgcGCCCGUGUGcuuuuacgUGguGCGCu -3' miRNA: 3'- uugCGCC---UGGGCAUACua------ACguCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 5 | 0.69 | 0.794458 |
Target: 5'- uACGCGGGgCCa-GUGAacaGCGGCGCg -3' miRNA: 3'- uUGCGCCUgGGcaUACUaa-CGUCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 124960 | 0.68 | 0.863166 |
Target: 5'- gAACGCGGcGCCgGUgAUGGggcGCuGCGCu -3' miRNA: 3'- -UUGCGCC-UGGgCA-UACUaa-CGuCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 30462 | 0.67 | 0.878289 |
Target: 5'- uGCGCGG-CUCGUuuuauaGAguugagGCGGCGCg -3' miRNA: 3'- uUGCGCCuGGGCAua----CUaa----CGUCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 75969 | 0.66 | 0.917957 |
Target: 5'- uGACGCGGcGCgCGU-UGGUgcaCGGCGCa -3' miRNA: 3'- -UUGCGCC-UGgGCAuACUAac-GUCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 6935 | 0.66 | 0.923699 |
Target: 5'- cAACGCGGaACCUGUGcGAa-GCGGUGg -3' miRNA: 3'- -UUGCGCC-UGGGCAUaCUaaCGUCGCg -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 78548 | 0.66 | 0.92919 |
Target: 5'- uACGCGGGCUCagcgGAUUG-AGCGUc -3' miRNA: 3'- uUGCGCCUGGGcauaCUAACgUCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 119117 | 1.1 | 0.002604 |
Target: 5'- aAACGCGGACCCGUAUGAUUGCAGCGCg -3' miRNA: 3'- -UUGCGCCUGGGCAUACUAACGUCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 70177 | 0.79 | 0.266384 |
Target: 5'- uACGCGGugCCGUggGAUUGcCGGCGg -3' miRNA: 3'- uUGCGCCugGGCAuaCUAAC-GUCGCg -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 100410 | 0.79 | 0.28647 |
Target: 5'- cGACGCGcGCCCGUGc-GUUGCGGCGCc -3' miRNA: 3'- -UUGCGCcUGGGCAUacUAACGUCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 92764 | 0.72 | 0.611458 |
Target: 5'- cAACGCGGACCCGUAU--UUGCuuaauuGUGa -3' miRNA: 3'- -UUGCGCCUGGGCAUAcuAACGu-----CGCg -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 130008 | 0.72 | 0.64302 |
Target: 5'- aAACGUGGGCUgagGUGAagGCGGCGCu -3' miRNA: 3'- -UUGCGCCUGGgcaUACUaaCGUCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 45186 | 0.68 | 0.838847 |
Target: 5'- uACGaCGGGCCCGUAaaGAUauuUGUGGcCGCg -3' miRNA: 3'- uUGC-GCCUGGGCAUa-CUA---ACGUC-GCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 32471 | 0.68 | 0.838847 |
Target: 5'- -cCGCGGACCa--AUGGUUaaGCAGCGa -3' miRNA: 3'- uuGCGCCUGGgcaUACUAA--CGUCGCg -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 99949 | 0.68 | 0.847162 |
Target: 5'- cGCGCGGcgACCuCGUGUacaGAUUGauauaaAGCGCu -3' miRNA: 3'- uUGCGCC--UGG-GCAUA---CUAACg-----UCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 33341 | 0.68 | 0.847162 |
Target: 5'- cGACGCGcaauuGACCCGUGUauaguaaaauggGAaUGCcGCGCu -3' miRNA: 3'- -UUGCGC-----CUGGGCAUA------------CUaACGuCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 24466 | 0.67 | 0.881204 |
Target: 5'- uGCGCGGACgCGgugGAcUGCaacaagccgucugucAGCGCg -3' miRNA: 3'- uUGCGCCUGgGCauaCUaACG---------------UCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 98194 | 0.66 | 0.911962 |
Target: 5'- aGGCGCugcuGGACCCGcuc----GCAGCGCc -3' miRNA: 3'- -UUGCG----CCUGGGCauacuaaCGUCGCG- -5' |
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22967 | 5' | -54.4 | NC_005137.2 | + | 55580 | 0.66 | 0.911962 |
Target: 5'- cAugGCGGACgCGg------GCGGCGCc -3' miRNA: 3'- -UugCGCCUGgGCauacuaaCGUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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