Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22970 | 5' | -51.6 | NC_005137.2 | + | 95755 | 0.71 | 0.827234 |
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Target: 5'- cAGCACGUC-GcGCACAGuGCGCGGGc -3' miRNA: 3'- cUCGUGCAGaC-CGUGUUuCGCGUUUc -5' |
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| 22970 | 5' | -51.6 | NC_005137.2 | + | 6998 | 0.71 | 0.81822 |
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Target: 5'- cGAGCG-GUCgccgGGCACAAcguGGCGCAc-- -3' miRNA: 3'- -CUCGUgCAGa---CCGUGUU---UCGCGUuuc -5' |
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| 22970 | 5' | -51.6 | NC_005137.2 | + | 75974 | 0.71 | 0.809017 |
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Target: 5'- cGGCGCGcgUUGGUGCAcGGCGCAcGGg -3' miRNA: 3'- cUCGUGCa-GACCGUGUuUCGCGUuUC- -5' |
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| 22970 | 5' | -51.6 | NC_005137.2 | + | 63270 | 0.72 | 0.770517 |
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Target: 5'- aAGcCGCGaCcGGCGCGAAGCGCAAGc -3' miRNA: 3'- cUC-GUGCaGaCCGUGUUUCGCGUUUc -5' |
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| 22970 | 5' | -51.6 | NC_005137.2 | + | 28215 | 0.76 | 0.569176 |
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Target: 5'- cAGC-CGUCUGGCGCAucccAGCGCGc-- -3' miRNA: 3'- cUCGuGCAGACCGUGUu---UCGCGUuuc -5' |
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| 22970 | 5' | -51.6 | NC_005137.2 | + | 120310 | 1.09 | 0.005675 |
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Target: 5'- cGAGCACGUCUGGCACAAAGCGCAAAGc -3' miRNA: 3'- -CUCGUGCAGACCGUGUUUCGCGUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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