Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22972 | 5' | -52.2 | NC_005137.2 | + | 41977 | 0.71 | 0.811698 |
Target: 5'- aGcGCUaaCAAACGCGUGGCGCGCcgUGa -3' miRNA: 3'- gCuCGA--GUUUGUGCGCCGUGUGa-ACa -5' |
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22972 | 5' | -52.2 | NC_005137.2 | + | 5255 | 0.71 | 0.793131 |
Target: 5'- --cGCUCgAAACGCGCGGCGCAa---- -3' miRNA: 3'- gcuCGAG-UUUGUGCGCCGUGUgaaca -5' |
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22972 | 5' | -52.2 | NC_005137.2 | + | 37605 | 0.73 | 0.681936 |
Target: 5'- uCGAGCgCAAAUACGUGGUGCGCg--- -3' miRNA: 3'- -GCUCGaGUUUGUGCGCCGUGUGaaca -5' |
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22972 | 5' | -52.2 | NC_005137.2 | + | 122498 | 0.75 | 0.618119 |
Target: 5'- gGGGCUUAuuuGACuuuuaGCGCGGCcACGCUUGUg -3' miRNA: 3'- gCUCGAGU---UUG-----UGCGCCG-UGUGAACA- -5' |
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22972 | 5' | -52.2 | NC_005137.2 | + | 110477 | 0.76 | 0.554688 |
Target: 5'- cCGGGCUCAAACACGC-GCACAa---- -3' miRNA: 3'- -GCUCGAGUUUGUGCGcCGUGUgaaca -5' |
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22972 | 5' | -52.2 | NC_005137.2 | + | 130650 | 1.09 | 0.005677 |
Target: 5'- gCGAGCUCAAACACGCGGCACACUUGUa -3' miRNA: 3'- -GCUCGAGUUUGUGCGCCGUGUGAACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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