Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22972 | 5' | -52.2 | NC_005137.2 | + | 33147 | 0.68 | 0.918356 |
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Target: 5'- -uGGUcugCAAACAUGUGcGCGCACUUGg -3' miRNA: 3'- gcUCGa--GUUUGUGCGC-CGUGUGAACa -5' |
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| 22972 | 5' | -52.2 | NC_005137.2 | + | 26802 | 0.66 | 0.9767 |
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Target: 5'- uCGuGUUCGcGCuGCGaCGcGCACGCUUGUa -3' miRNA: 3'- -GCuCGAGUuUG-UGC-GC-CGUGUGAACA- -5' |
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| 22972 | 5' | -52.2 | NC_005137.2 | + | 18644 | 0.66 | 0.974058 |
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Target: 5'- uGGGCUUAAACuugACGuCGGCACAg---- -3' miRNA: 3'- gCUCGAGUUUG---UGC-GCCGUGUgaaca -5' |
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| 22972 | 5' | -52.2 | NC_005137.2 | + | 6997 | 0.67 | 0.953531 |
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Target: 5'- aCGAGCggucgcCGGGCacaACGUGGCGCACg--- -3' miRNA: 3'- -GCUCGa-----GUUUG---UGCGCCGUGUGaaca -5' |
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| 22972 | 5' | -52.2 | NC_005137.2 | + | 5255 | 0.71 | 0.793131 |
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Target: 5'- --cGCUCgAAACGCGCGGCGCAa---- -3' miRNA: 3'- gcuCGAG-UUUGUGCGCCGUGUgaaca -5' |
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| 22972 | 5' | -52.2 | NC_005137.2 | + | 2591 | 0.66 | 0.974058 |
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Target: 5'- uCGGGCgCGGGCACGuCGGC-CACcgcgUGa -3' miRNA: 3'- -GCUCGaGUUUGUGC-GCCGuGUGa---ACa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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