Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22973 | 5' | -54.1 | NC_005137.2 | + | 96559 | 0.76 | 0.45204 |
Target: 5'- ----cGCCGcGCGGGUaGCGGuCGGCGCGg -3' miRNA: 3'- aaaaaCGGC-CGUCCA-UGCU-GCUGCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 4599 | 0.68 | 0.848556 |
Target: 5'- ----cGaCGGCgcgaAGGUGCG-CGACGCGg -3' miRNA: 3'- aaaaaCgGCCG----UCCAUGCuGCUGCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 50767 | 0.68 | 0.872107 |
Target: 5'- ---gUGCCGGCGuGUAUGAguACGCGu -3' miRNA: 3'- aaaaACGGCCGUcCAUGCUgcUGCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 110965 | 0.67 | 0.90676 |
Target: 5'- ----cGUCGGCGGGcgGCGuCGAuUGCGa -3' miRNA: 3'- aaaaaCGGCCGUCCa-UGCuGCU-GCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 10881 | 0.66 | 0.935245 |
Target: 5'- ---gUGCUGGCcgacGcGUGCG-CGAUGCGg -3' miRNA: 3'- aaaaACGGCCGu---C-CAUGCuGCUGCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 130780 | 0.66 | 0.935245 |
Target: 5'- cUUUUGCUGGCuuuGU-CGGCG-CGCGu -3' miRNA: 3'- aAAAACGGCCGuc-CAuGCUGCuGCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 115237 | 0.67 | 0.912959 |
Target: 5'- gUUUUGgUGGgAGGUcgacgaacacAUGGCGACGCu -3' miRNA: 3'- aAAAACgGCCgUCCA----------UGCUGCUGCGc -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 71363 | 0.66 | 0.940186 |
Target: 5'- ----cGUCGGCcGcGUugacuguCGGCGACGCGg -3' miRNA: 3'- aaaaaCGGCCGuC-CAu------GCUGCUGCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 34863 | 0.66 | 0.940186 |
Target: 5'- ----cGCaCGGCGGGcaACG-CGAUGCGc -3' miRNA: 3'- aaaaaCG-GCCGUCCa-UGCuGCUGCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 78804 | 0.66 | 0.935245 |
Target: 5'- -gUUUGCgGaGCGGGaGCGGCGuCGuCGg -3' miRNA: 3'- aaAAACGgC-CGUCCaUGCUGCuGC-GC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 42641 | 0.66 | 0.918909 |
Target: 5'- -cUUUGuuGGUGGGUACaugGACuacuuuGGCGCGa -3' miRNA: 3'- aaAAACggCCGUCCAUG---CUG------CUGCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 7308 | 0.66 | 0.918909 |
Target: 5'- -cUUUGCCcgGGCAGGaUGCGGucaaagugcUGGCGCc -3' miRNA: 3'- aaAAACGG--CCGUCC-AUGCU---------GCUGCGc -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 130930 | 1.06 | 0.005844 |
Target: 5'- cUUUUUGCCGGCAGGUACGACGACGCGc -3' miRNA: 3'- -AAAAACGGCCGUCCAUGCUGCUGCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 45104 | 0.67 | 0.90676 |
Target: 5'- ---cUGCCacucGCGGGcGCG-CGACGCGu -3' miRNA: 3'- aaaaACGGc---CGUCCaUGCuGCUGCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 125017 | 0.67 | 0.893619 |
Target: 5'- -----aCCGGCGaucGGuUGCGGCGugGCGc -3' miRNA: 3'- aaaaacGGCCGU---CC-AUGCUGCugCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 116456 | 0.67 | 0.886684 |
Target: 5'- ---gUGCCGGCAGaaGCGAaGGCGUu -3' miRNA: 3'- aaaaACGGCCGUCcaUGCUgCUGCGc -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 17829 | 0.68 | 0.840281 |
Target: 5'- gUUUUGgUGGCAGGUAuuguUGGCGuCGUGu -3' miRNA: 3'- aAAAACgGCCGUCCAU----GCUGCuGCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 103810 | 0.69 | 0.823141 |
Target: 5'- --gUUGCUGGU--GUGCGGCGGCGgGu -3' miRNA: 3'- aaaAACGGCCGucCAUGCUGCUGCgC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 97391 | 0.69 | 0.822264 |
Target: 5'- --gUUGCCGcgcaaauaaguuuGCAGGUugGGCacguacaccgGGCGCGg -3' miRNA: 3'- aaaAACGGC-------------CGUCCAugCUG----------CUGCGC- -5' |
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22973 | 5' | -54.1 | NC_005137.2 | + | 123056 | 0.69 | 0.814292 |
Target: 5'- ----cGCCGGUcgAGGUcGCGGCG-CGCa -3' miRNA: 3'- aaaaaCGGCCG--UCCA-UGCUGCuGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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