Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2298 | 5' | -54.6 | NC_001408.1 | + | 1203 | 0.66 | 0.195017 |
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Target: 5'- aAGUGGAAGcgcucauGUCCUCCCcGuuGC-UGCc -3' miRNA: 3'- -UCGCCUUC-------CAGGAGGGcUuuCGuACG- -5' |
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| 2298 | 5' | -54.6 | NC_001408.1 | + | 985 | 0.67 | 0.166882 |
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Target: 5'- gGGCGGAgccagggacgcGGGUCUggCCCcuGGGCcgGCc -3' miRNA: 3'- -UCGCCU-----------UCCAGGa-GGGcuUUCGuaCG- -5' |
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| 2298 | 5' | -54.6 | NC_001408.1 | + | 676 | 0.67 | 0.161607 |
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Target: 5'- gAG-GGAGGGUCuCUCCCccAGGUccggaGUGCa -3' miRNA: 3'- -UCgCCUUCCAG-GAGGGcuUUCG-----UACG- -5' |
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| 2298 | 5' | -54.6 | NC_001408.1 | + | 1868 | 0.7 | 0.098759 |
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Target: 5'- gGGuCGGguGGUCgUgCCCGAGGGCucUGCu -3' miRNA: 3'- -UC-GCCuuCCAGgA-GGGCUUUCGu-ACG- -5' |
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| 2298 | 5' | -54.6 | NC_001408.1 | + | 1726 | 0.72 | 0.061561 |
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Target: 5'- cGUGGAGGGUgCUgCCCGuauAAGC-UGCu -3' miRNA: 3'- uCGCCUUCCAgGA-GGGCu--UUCGuACG- -5' |
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| 2298 | 5' | -54.6 | NC_001408.1 | + | 3694 | 1.13 | 2.8e-05 |
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Target: 5'- gAGCGGAAGGUCCUCCCGAAAGCAUGCa -3' miRNA: 3'- -UCGCCUUCCAGGAGGGCUUUCGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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