miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2298 5' -54.6 NC_001408.1 + 3694 1.13 0.000028
Target:  5'- gAGCGGAAGGUCCUCCCGAAAGCAUGCa -3'
miRNA:   3'- -UCGCCUUCCAGGAGGGCUUUCGUACG- -5'
2298 5' -54.6 NC_001408.1 + 1726 0.72 0.061561
Target:  5'- cGUGGAGGGUgCUgCCCGuauAAGC-UGCu -3'
miRNA:   3'- uCGCCUUCCAgGA-GGGCu--UUCGuACG- -5'
2298 5' -54.6 NC_001408.1 + 1868 0.7 0.098759
Target:  5'- gGGuCGGguGGUCgUgCCCGAGGGCucUGCu -3'
miRNA:   3'- -UC-GCCuuCCAGgA-GGGCUUUCGu-ACG- -5'
2298 5' -54.6 NC_001408.1 + 676 0.67 0.161607
Target:  5'- gAG-GGAGGGUCuCUCCCccAGGUccggaGUGCa -3'
miRNA:   3'- -UCgCCUUCCAG-GAGGGcuUUCG-----UACG- -5'
2298 5' -54.6 NC_001408.1 + 985 0.67 0.166882
Target:  5'- gGGCGGAgccagggacgcGGGUCUggCCCcuGGGCcgGCc -3'
miRNA:   3'- -UCGCCU-----------UCCAGGa-GGGcuUUCGuaCG- -5'
2298 5' -54.6 NC_001408.1 + 1203 0.66 0.195017
Target:  5'- aAGUGGAAGcgcucauGUCCUCCCcGuuGC-UGCc -3'
miRNA:   3'- -UCGCCUUC-------CAGGAGGGcUuuCGuACG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.