miRNA: 3'- gUCACUAUUuuauUCUC-ACAUACGAc -5'
miRNA: 3'- -GUCACUAU-------UUUAU-UCUC-ACAUACGAc -5'
miRNA: 3'- gUCACUAUU-UUAUUCUCACAuACGac -5'
miRNA: 3'- -GUCACUAUUUUAUUCUCACAUACGAC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22983 | 5' | -40.9 | NC_005147.1 | + | 15563 | 0.67 | 0.999375 |
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Target: 5'- gAGUGAUGAu---GGGGuUGUGUGUUa -3' miRNA: 3'- gUCACUAUUuuauUCUC-ACAUACGAc -5' |
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| 22983 | 5' | -40.9 | NC_005147.1 | + | 10164 | 0.69 | 0.996809 |
|
Target: 5'- aCAGUGAUGucuuaucAAAUGcGGGGuUGUAUGCUu -3' miRNA: 3'- -GUCACUAU-------UUUAU-UCUC-ACAUACGAc -5' |
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| 22983 | 5' | -40.9 | NC_005147.1 | + | 20391 | 0.78 | 0.756544 |
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Target: 5'- gAGUGGUGGuAGUAAGAGUGUuUGCa- -3' miRNA: 3'- gUCACUAUU-UUAUUCUCACAuACGac -5' |
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| 22983 | 5' | -40.9 | NC_005147.1 | + | 20414 | 1.12 | 0.01176 |
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Target: 5'- uCAGUGAUAAAAUAAGAGUGUAUGCUGg -3' miRNA: 3'- -GUCACUAUUUUAUUCUCACAUACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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