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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22998 | 5' | -51.9 | NC_005157.1 | + | 2220 | 0.66 | 0.152231 |
Target: 5'- aUGGCgGUCAuaacaccagucauggUGCGUCGGG-UGAGAUCa -3' miRNA: 3'- -ACUGgCGGU---------------ACGCGGUUCaAUUCUGG- -5' |
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22998 | 5' | -51.9 | NC_005157.1 | + | 1994 | 0.68 | 0.105302 |
Target: 5'- -uGCUGCCAUa-GCCAaauucacugGGUUGGGGCCa -3' miRNA: 3'- acUGGCGGUAcgCGGU---------UCAAUUCUGG- -5' |
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22998 | 5' | -51.9 | NC_005157.1 | + | 2207 | 1.14 | 1.4e-05 |
Target: 5'- aUGACCGCCAUGCGCCAAGUUAAGACCa -3' miRNA: 3'- -ACUGGCGGUACGCGGUUCAAUUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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