Results 1 - 2 of 2 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2302 | 5' | -59 | NC_001408.1 | + | 340 | 0.69 | 0.050872 |
Target: 5'- ---aGGCaCGUCUUUGGuCGGCCUGCg -3' miRNA: 3'- agcaCCGgGCAGAGACUcGUCGGACG- -5' |
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2302 | 5' | -59 | NC_001408.1 | + | 2067 | 1.11 | 1.3e-05 |
Target: 5'- gUCGUGGCCCGUCUCUGAGCAGCCUGCc -3' miRNA: 3'- -AGCACCGGGCAGAGACUCGUCGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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