Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23022 | 5' | -60.7 | NC_005178.1 | + | 9427 | 0.66 | 0.31841 |
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Target: 5'- -cCUGCUGCGGuuccucaccGCGaGcgCgCCGUGCCAg -3' miRNA: 3'- gaGACGACGCU---------CGC-CaaG-GGCGCGGU- -5' |
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| 23022 | 5' | -60.7 | NC_005178.1 | + | 9601 | 0.67 | 0.267299 |
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Target: 5'- gCUUcGCUcGUuGGC-GUUCCCGCGCCAg -3' miRNA: 3'- -GAGaCGA-CGcUCGcCAAGGGCGCGGU- -5' |
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| 23022 | 5' | -60.7 | NC_005178.1 | + | 8422 | 0.68 | 0.235 |
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Target: 5'- -gCUGUUGCGcGCGG-UUgCGUGCCAu -3' miRNA: 3'- gaGACGACGCuCGCCaAGgGCGCGGU- -5' |
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| 23022 | 5' | -60.7 | NC_005178.1 | + | 13148 | 0.69 | 0.206042 |
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Target: 5'- ---aGCUucauccGUGAGCGGUUCCCGCaGCa- -3' miRNA: 3'- gagaCGA------CGCUCGCCAAGGGCG-CGgu -5' |
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| 23022 | 5' | -60.7 | NC_005178.1 | + | 16190 | 0.76 | 0.05674 |
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Target: 5'- -cCUGCUGCGAGCGGUgaccggCCuCG-GCCAu -3' miRNA: 3'- gaGACGACGCUCGCCAa-----GG-GCgCGGU- -5' |
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| 23022 | 5' | -60.7 | NC_005178.1 | + | 2043 | 1.08 | 0.000207 |
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Target: 5'- cCUCUGCUGCGAGCGGUUCCCGCGCCAc -3' miRNA: 3'- -GAGACGACGCUCGCCAAGGGCGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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