miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23022 5' -60.7 NC_005178.1 + 9427 0.66 0.31841
Target:  5'- -cCUGCUGCGGuuccucaccGCGaGcgCgCCGUGCCAg -3'
miRNA:   3'- gaGACGACGCU---------CGC-CaaG-GGCGCGGU- -5'
23022 5' -60.7 NC_005178.1 + 9601 0.67 0.267299
Target:  5'- gCUUcGCUcGUuGGC-GUUCCCGCGCCAg -3'
miRNA:   3'- -GAGaCGA-CGcUCGcCAAGGGCGCGGU- -5'
23022 5' -60.7 NC_005178.1 + 8422 0.68 0.235
Target:  5'- -gCUGUUGCGcGCGG-UUgCGUGCCAu -3'
miRNA:   3'- gaGACGACGCuCGCCaAGgGCGCGGU- -5'
23022 5' -60.7 NC_005178.1 + 13148 0.69 0.206042
Target:  5'- ---aGCUucauccGUGAGCGGUUCCCGCaGCa- -3'
miRNA:   3'- gagaCGA------CGCUCGCCAAGGGCG-CGgu -5'
23022 5' -60.7 NC_005178.1 + 16190 0.76 0.05674
Target:  5'- -cCUGCUGCGAGCGGUgaccggCCuCG-GCCAu -3'
miRNA:   3'- gaGACGACGCUCGCCAa-----GG-GCgCGGU- -5'
23022 5' -60.7 NC_005178.1 + 2043 1.08 0.000207
Target:  5'- cCUCUGCUGCGAGCGGUUCCCGCGCCAc -3'
miRNA:   3'- -GAGACGACGCUCGCCAAGGGCGCGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.