Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23025 | 5' | -53.4 | NC_005178.1 | + | 14573 | 0.66 | 0.768468 |
Target: 5'- gGGUUuuUCaGCaGGCGCGACaGGACcGAGu -3' miRNA: 3'- -CCAAuuGG-CG-CUGCGCUG-CCUGuCUC- -5' |
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23025 | 5' | -53.4 | NC_005178.1 | + | 24119 | 0.66 | 0.767428 |
Target: 5'- aGGUcgUGACCaagGCGGCgggcuccGUGACGGGC-GAGg -3' miRNA: 3'- -CCA--AUUGG---CGCUG-------CGCUGCCUGuCUC- -5' |
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23025 | 5' | -53.4 | NC_005178.1 | + | 10761 | 0.66 | 0.747412 |
Target: 5'- cGG-UGAgUGCGACGCGggauuaGCGGAaGGGGu -3' miRNA: 3'- -CCaAUUgGCGCUGCGC------UGCCUgUCUC- -5' |
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23025 | 5' | -53.4 | NC_005178.1 | + | 7935 | 0.66 | 0.725865 |
Target: 5'- cGGc--GCCGCGcACaGUcACGGACGGGGg -3' miRNA: 3'- -CCaauUGGCGC-UG-CGcUGCCUGUCUC- -5' |
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23025 | 5' | -53.4 | NC_005178.1 | + | 28877 | 0.66 | 0.724776 |
Target: 5'- ----cACCGCGAacccagaccugguCGCGGCuGGGCAGGc -3' miRNA: 3'- ccaauUGGCGCU-------------GCGCUG-CCUGUCUc -5' |
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23025 | 5' | -53.4 | NC_005178.1 | + | 21301 | 0.67 | 0.692839 |
Target: 5'- cGGUUcgacGCCGaUGAUGCGGCcGGcCGGGGa -3' miRNA: 3'- -CCAAu---UGGC-GCUGCGCUG-CCuGUCUC- -5' |
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23025 | 5' | -53.4 | NC_005178.1 | + | 14881 | 0.67 | 0.670493 |
Target: 5'- cGGUUGACCcagaacGUGAUGCaGGCGGcgaAGGGg -3' miRNA: 3'- -CCAAUUGG------CGCUGCG-CUGCCug-UCUC- -5' |
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23025 | 5' | -53.4 | NC_005178.1 | + | 31361 | 0.67 | 0.658132 |
Target: 5'- cGGUgGACCGCGA-GCGGCagcuacuGGACAuGAc -3' miRNA: 3'- -CCAaUUGGCGCUgCGCUG-------CCUGU-CUc -5' |
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23025 | 5' | -53.4 | NC_005178.1 | + | 28611 | 0.69 | 0.558223 |
Target: 5'- -----cCCgGUGGCGCGGCGGACGGc- -3' miRNA: 3'- ccaauuGG-CGCUGCGCUGCCUGUCuc -5' |
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23025 | 5' | -53.4 | NC_005178.1 | + | 17663 | 0.7 | 0.514529 |
Target: 5'- aGG-UGACCGUcgaGACGgGcguagacaaGCGGACAGGGg -3' miRNA: 3'- -CCaAUUGGCG---CUGCgC---------UGCCUGUCUC- -5' |
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23025 | 5' | -53.4 | NC_005178.1 | + | 11021 | 0.74 | 0.323842 |
Target: 5'- cGGUgu-CgGUGACGCGGgGGGCAGGa -3' miRNA: 3'- -CCAauuGgCGCUGCGCUgCCUGUCUc -5' |
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23025 | 5' | -53.4 | NC_005178.1 | + | 9113 | 0.74 | 0.315803 |
Target: 5'- cGG-UGACCGCGAgCGCGGCGcGGCAu-- -3' miRNA: 3'- -CCaAUUGGCGCU-GCGCUGC-CUGUcuc -5' |
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23025 | 5' | -53.4 | NC_005178.1 | + | 5301 | 1.11 | 0.00093 |
Target: 5'- aGGUUAACCGCGACGCGACGGACAGAGc -3' miRNA: 3'- -CCAAUUGGCGCUGCGCUGCCUGUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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