Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23029 | 3' | -63.7 | NC_005178.1 | + | 10326 | 0.66 | 0.216867 |
Target: 5'- aGGGCCGGGCgcggaggggcgaagCGcgCCgggcugcGCUGGCCgAGc -3' miRNA: 3'- -UUCGGCCCG--------------GCuaGGa------CGACCGGgUC- -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 35669 | 0.66 | 0.211256 |
Target: 5'- -cGCCGGccucacauccagcaGuuGGUCCUauccGCUGGCCgAGg -3' miRNA: 3'- uuCGGCC--------------CggCUAGGA----CGACCGGgUC- -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 11393 | 0.66 | 0.207403 |
Target: 5'- cAGCa-GGCCGA-CC-GCUGGCuCCAGu -3' miRNA: 3'- uUCGgcCCGGCUaGGaCGACCG-GGUC- -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 23744 | 0.66 | 0.202005 |
Target: 5'- -cGCCGaaGCCGAguUCCUGCUGcGCCg-- -3' miRNA: 3'- uuCGGCc-CGGCU--AGGACGAC-CGGguc -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 11785 | 0.66 | 0.202005 |
Target: 5'- -cGCCGcaGGCCGG-CCUGCacaUGGaCCAGg -3' miRNA: 3'- uuCGGC--CCGGCUaGGACG---ACCgGGUC- -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 16801 | 0.67 | 0.186538 |
Target: 5'- -uGCU-GGCCag-CCUGCcGGCCCAGg -3' miRNA: 3'- uuCGGcCCGGcuaGGACGaCCGGGUC- -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 36040 | 0.67 | 0.181619 |
Target: 5'- gGGGCCGGGgCGAaCCagaaccGCuUGGCCCGc -3' miRNA: 3'- -UUCGGCCCgGCUaGGa-----CG-ACCGGGUc -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 10933 | 0.67 | 0.172124 |
Target: 5'- -cGCCGaGUCGAUCCgGCgcGGCCCGc -3' miRNA: 3'- uuCGGCcCGGCUAGGaCGa-CCGGGUc -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 9449 | 0.67 | 0.163075 |
Target: 5'- uAGCCGGGaCGGUCUgccggacgUGgUGGUCCGGu -3' miRNA: 3'- uUCGGCCCgGCUAGG--------ACgACCGGGUC- -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 25457 | 0.67 | 0.163075 |
Target: 5'- --uCCGGGCCG--CCUGCUGGUCg-- -3' miRNA: 3'- uucGGCCCGGCuaGGACGACCGGguc -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 16046 | 0.67 | 0.158713 |
Target: 5'- uAG-CGGGCUGuUCCgcgUGCUGGCCUGGc -3' miRNA: 3'- uUCgGCCCGGCuAGG---ACGACCGGGUC- -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 26895 | 0.68 | 0.142301 |
Target: 5'- cAGCCcaacGGGCCGAagacagCCccGCUGGCCaCAGc -3' miRNA: 3'- uUCGG----CCCGGCUa-----GGa-CGACCGG-GUC- -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 17268 | 0.68 | 0.138448 |
Target: 5'- --cCUGcGGCCGAUCCUGgaGGCCaaaGGc -3' miRNA: 3'- uucGGC-CCGGCUAGGACgaCCGGg--UC- -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 16717 | 0.8 | 0.015096 |
Target: 5'- uGAGCCGGcGCCcuGGUCCUcgcaucgcugcGCUGGCCCAGg -3' miRNA: 3'- -UUCGGCC-CGG--CUAGGA-----------CGACCGGGUC- -5' |
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23029 | 3' | -63.7 | NC_005178.1 | + | 8970 | 1.06 | 0.000114 |
Target: 5'- gAAGCCGGGCCGAUCCUGCUGGCCCAGg -3' miRNA: 3'- -UUCGGCCCGGCUAGGACGACCGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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