Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23030 | 3' | -55.7 | NC_005178.1 | + | 29143 | 0.66 | 0.551947 |
Target: 5'- cAGCCCgcggGUUC-AGGCGCUgcUGugGAa -3' miRNA: 3'- uUCGGGa---CAAGcUUCGCGA--GCugCUa -5' |
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23030 | 3' | -55.7 | NC_005178.1 | + | 18480 | 0.66 | 0.551947 |
Target: 5'- uGAGCCCcGUUacCGuugcAGCGCgagCGGCGAa -3' miRNA: 3'- -UUCGGGaCAA--GCu---UCGCGa--GCUGCUa -5' |
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23030 | 3' | -55.7 | NC_005178.1 | + | 31328 | 0.66 | 0.551947 |
Target: 5'- -cGCUUUGcaggauauuugUCGAggcgcucaGGCGCUCGACGAUg -3' miRNA: 3'- uuCGGGACa----------AGCU--------UCGCGAGCUGCUA- -5' |
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23030 | 3' | -55.7 | NC_005178.1 | + | 22720 | 0.66 | 0.540743 |
Target: 5'- -cGCCCUGggcuucgaugaUCGGAGCguuguGCUCGGCGu- -3' miRNA: 3'- uuCGGGACa----------AGCUUCG-----CGAGCUGCua -5' |
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23030 | 3' | -55.7 | NC_005178.1 | + | 15832 | 0.66 | 0.518577 |
Target: 5'- -uGCCCUccaggUCGAgcAGCaGCUCGugGAg -3' miRNA: 3'- uuCGGGAca---AGCU--UCG-CGAGCugCUa -5' |
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23030 | 3' | -55.7 | NC_005178.1 | + | 9575 | 0.66 | 0.507629 |
Target: 5'- -uGCCCUGcgccgCGcuGCGCUgGGCGGUc -3' miRNA: 3'- uuCGGGACaa---GCuuCGCGAgCUGCUA- -5' |
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23030 | 3' | -55.7 | NC_005178.1 | + | 4239 | 0.66 | 0.4957 |
Target: 5'- gAGGCCCgcga-GAAGCGCgcccuggcucaccUCGACGAg -3' miRNA: 3'- -UUCGGGacaagCUUCGCG-------------AGCUGCUa -5' |
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23030 | 3' | -55.7 | NC_005178.1 | + | 28913 | 0.67 | 0.486036 |
Target: 5'- gAGGaaCUGgaCGAGGCGCUCGACc-- -3' miRNA: 3'- -UUCggGACaaGCUUCGCGAGCUGcua -5' |
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23030 | 3' | -55.7 | NC_005178.1 | + | 27609 | 0.67 | 0.444232 |
Target: 5'- cGGCCCUGUUCGuuGaGCUgGAgGAc -3' miRNA: 3'- uUCGGGACAAGCuuCgCGAgCUgCUa -5' |
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23030 | 3' | -55.7 | NC_005178.1 | + | 7076 | 0.72 | 0.21722 |
Target: 5'- cGGCCCUGggCGAGGCGgugacCUCGGuCGAg -3' miRNA: 3'- uUCGGGACaaGCUUCGC-----GAGCU-GCUa -5' |
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23030 | 3' | -55.7 | NC_005178.1 | + | 10541 | 1.03 | 0.001246 |
Target: 5'- cAAGCCCUGUUCGAAGCGCUCGACGAUc -3' miRNA: 3'- -UUCGGGACAAGCUUCGCGAGCUGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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