Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23031 | 3' | -54.9 | NC_005178.1 | + | 1535 | 0.66 | 0.580973 |
Target: 5'- aGGUCGCCG-CCCGCaugUCGcGCcuguccGGCGa -3' miRNA: 3'- aUUAGCGGCaGGGCG---AGUuUGu-----CCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 2587 | 0.69 | 0.450808 |
Target: 5'- aUGGUCGCC-UCCaguaGuCUCAuGGCGGGCGa -3' miRNA: 3'- -AUUAGCGGcAGGg---C-GAGU-UUGUCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 2810 | 0.68 | 0.471491 |
Target: 5'- cUGGUCGCCGaugCCCGC-CAGGgGGuGCu -3' miRNA: 3'- -AUUAGCGGCa--GGGCGaGUUUgUC-CGc -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 4128 | 0.71 | 0.338288 |
Target: 5'- cUGGUCGCCGUggaauuccUCCaGCUC--GCGGGCGa -3' miRNA: 3'- -AUUAGCGGCA--------GGG-CGAGuuUGUCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 4595 | 0.71 | 0.321634 |
Target: 5'- ---gCGgCGUCCgGCUCGAuggGCAGGCc -3' miRNA: 3'- auuaGCgGCAGGgCGAGUU---UGUCCGc -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 5614 | 0.68 | 0.461089 |
Target: 5'- gGAUCGCCucgacGUagCCGCccaggcgCAGGCAGGCGa -3' miRNA: 3'- aUUAGCGG-----CAg-GGCGa------GUUUGUCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 7595 | 0.69 | 0.410999 |
Target: 5'- -cGUCuaCGUCCCGCagacccgCGAGCAGGUc -3' miRNA: 3'- auUAGcgGCAGGGCGa------GUUUGUCCGc -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 11486 | 1.07 | 0.000912 |
Target: 5'- aUAAUCGCCGUCCCGCUCAAACAGGCGc -3' miRNA: 3'- -AUUAGCGGCAGGGCGAGUUUGUCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 12297 | 0.77 | 0.13778 |
Target: 5'- uUGGUCGCUGUcggCCCGCUUGGcCAGGCGc -3' miRNA: 3'- -AUUAGCGGCA---GGGCGAGUUuGUCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 13611 | 0.67 | 0.569683 |
Target: 5'- ---aCGCUGaCCCGC-CGcAUAGGCGa -3' miRNA: 3'- auuaGCGGCaGGGCGaGUuUGUCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 13949 | 0.69 | 0.450808 |
Target: 5'- ---gCGCUGgCCCGUUCAcgcCGGGCGg -3' miRNA: 3'- auuaGCGGCaGGGCGAGUuu-GUCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 20174 | 0.67 | 0.547276 |
Target: 5'- --cUCGCCGUCuUCGaUCAGcuucgcggcGCGGGCGg -3' miRNA: 3'- auuAGCGGCAG-GGCgAGUU---------UGUCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 22850 | 0.71 | 0.3056 |
Target: 5'- --cUCGCCGUaCUGCUCGAcCAGGuCGg -3' miRNA: 3'- auuAGCGGCAgGGCGAGUUuGUCC-GC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 24479 | 0.67 | 0.547276 |
Target: 5'- aGGUCGCgacCGaCCCGCUCcagGAGCuGGCa -3' miRNA: 3'- aUUAGCG---GCaGGGCGAG---UUUGuCCGc -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 25127 | 0.73 | 0.261201 |
Target: 5'- cUGGUCGCgcuggUGUCCUGCUgCGAcgagGCAGGCGa -3' miRNA: 3'- -AUUAGCG-----GCAGGGCGA-GUU----UGUCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 29124 | 0.71 | 0.3056 |
Target: 5'- ---aCGCCGUgCCGCcCAu-CAGGCGa -3' miRNA: 3'- auuaGCGGCAgGGCGaGUuuGUCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 29643 | 0.66 | 0.60368 |
Target: 5'- --uUCuaCGUCCCaacGCUCAAcuCGGGCGc -3' miRNA: 3'- auuAGcgGCAGGG---CGAGUUu-GUCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 31083 | 0.68 | 0.482011 |
Target: 5'- ---aUGCCGucugcaucuUCCCGCUCGAu--GGCGg -3' miRNA: 3'- auuaGCGGC---------AGGGCGAGUUuguCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 36477 | 0.68 | 0.482011 |
Target: 5'- gUAAUCGUCGcCCCGUUCc-AC-GGCGa -3' miRNA: 3'- -AUUAGCGGCaGGGCGAGuuUGuCCGC- -5' |
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23031 | 3' | -54.9 | NC_005178.1 | + | 36527 | 0.66 | 0.592309 |
Target: 5'- ---cCGCCGUCCCGC-CGuuuguGGUGu -3' miRNA: 3'- auuaGCGGCAGGGCGaGUuugu-CCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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