Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23033 | 3' | -56.1 | NC_005178.1 | + | 13641 | 1.07 | 0.000622 |
Target: 5'- cAACUGAACAGCGCCCACGCGGAUCAGa -3' miRNA: 3'- -UUGACUUGUCGCGGGUGCGCCUAGUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 16934 | 0.76 | 0.127935 |
Target: 5'- gGAC--GACAGCGCCCugGCGGAUg-- -3' miRNA: 3'- -UUGacUUGUCGCGGGugCGCCUAguc -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 28668 | 0.74 | 0.174625 |
Target: 5'- -uCUGAGCGGCGCCCAUGCuugggcgugguagGGAUggCGGg -3' miRNA: 3'- uuGACUUGUCGCGGGUGCG-------------CCUA--GUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 11768 | 0.73 | 0.190448 |
Target: 5'- -gUUGGACGGCGCCCA-GCGagcGAUCAGc -3' miRNA: 3'- uuGACUUGUCGCGGGUgCGC---CUAGUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 30816 | 0.72 | 0.21862 |
Target: 5'- -cCUGGGcCGGCGUCUACGCGGcgcAUCAGc -3' miRNA: 3'- uuGACUU-GUCGCGGGUGCGCC---UAGUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 3995 | 0.72 | 0.246286 |
Target: 5'- gGGCUGAaacccuccgcuucguGCAGCGCCCAgGCuucguuggGGGUCAu -3' miRNA: 3'- -UUGACU---------------UGUCGCGGGUgCG--------CCUAGUc -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 5544 | 0.72 | 0.224665 |
Target: 5'- ---gGAGCGGCGCgCCACGCGGuagaCGGu -3' miRNA: 3'- uugaCUUGUCGCG-GGUGCGCCua--GUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 20600 | 0.72 | 0.218023 |
Target: 5'- gAGCUGggUgacagcuucaacgGGCGCCCACuucgcCGGGUCGGg -3' miRNA: 3'- -UUGACuuG-------------UCGCGGGUGc----GCCUAGUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 19096 | 0.72 | 0.212714 |
Target: 5'- uACUGAGUAGUGCCCGacUGGAUCAGg -3' miRNA: 3'- uUGACUUGUCGCGGGUgcGCCUAGUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 13776 | 0.7 | 0.308657 |
Target: 5'- gGACUGGAUugcuGGUGCCUACGgCGGcAUCAa -3' miRNA: 3'- -UUGACUUG----UCGCGGGUGC-GCC-UAGUc -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 25460 | 0.69 | 0.358017 |
Target: 5'- cGCUGGAC-GCGCUgGCGgaaaguuCGGAUCGGa -3' miRNA: 3'- uUGACUUGuCGCGGgUGC-------GCCUAGUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 16376 | 0.68 | 0.414591 |
Target: 5'- gGGCg--GCGGCGCCUACGCccuGGggCAGg -3' miRNA: 3'- -UUGacuUGUCGCGGGUGCG---CCuaGUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 35750 | 0.68 | 0.386083 |
Target: 5'- -cCUGcGCAGUGUCCGCGaCGGcAUCAa -3' miRNA: 3'- uuGACuUGUCGCGGGUGC-GCC-UAGUc -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 7882 | 0.68 | 0.386083 |
Target: 5'- uGACUGugcGCGGCGCCgAUGCGG-UCc- -3' miRNA: 3'- -UUGACu--UGUCGCGGgUGCGCCuAGuc -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 29477 | 0.68 | 0.403988 |
Target: 5'- aAACcGAGcCAGCGCCCGgcgggauCGCGGAgauagCAGc -3' miRNA: 3'- -UUGaCUU-GUCGCGGGU-------GCGCCUa----GUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 13934 | 0.68 | 0.414591 |
Target: 5'- cGCcGGGCGGCGCUgGCcuuCGGGUCGGg -3' miRNA: 3'- uUGaCUUGUCGCGGgUGc--GCCUAGUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 13527 | 0.67 | 0.474233 |
Target: 5'- gAACUGAGCaauuucggcuuccAGCucGCCUAUgcgGCGGGUCAGc -3' miRNA: 3'- -UUGACUUG-------------UCG--CGGGUG---CGCCUAGUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 16896 | 0.67 | 0.444355 |
Target: 5'- gAGCUGcuCGGCGCCCugGCcGAggCAu -3' miRNA: 3'- -UUGACuuGUCGCGGGugCGcCUa-GUc -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 20795 | 0.67 | 0.434299 |
Target: 5'- cAGCUGGACgaGGcCGCCCuCGCGGuuugcUCGGc -3' miRNA: 3'- -UUGACUUG--UC-GCGGGuGCGCCu----AGUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 10724 | 0.67 | 0.444355 |
Target: 5'- cGGCUGAGaCAGgGUCgGCGCGGAg--- -3' miRNA: 3'- -UUGACUU-GUCgCGGgUGCGCCUaguc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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