Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23033 | 3' | -56.1 | NC_005178.1 | + | 11768 | 0.73 | 0.190448 |
Target: 5'- -gUUGGACGGCGCCCA-GCGagcGAUCAGc -3' miRNA: 3'- uuGACUUGUCGCGGGUgCGC---CUAGUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 28668 | 0.74 | 0.174625 |
Target: 5'- -uCUGAGCGGCGCCCAUGCuugggcgugguagGGAUggCGGg -3' miRNA: 3'- uuGACUUGUCGCGGGUGCG-------------CCUA--GUC- -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 16934 | 0.76 | 0.127935 |
Target: 5'- gGAC--GACAGCGCCCugGCGGAUg-- -3' miRNA: 3'- -UUGacUUGUCGCGGGugCGCCUAguc -5' |
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23033 | 3' | -56.1 | NC_005178.1 | + | 13641 | 1.07 | 0.000622 |
Target: 5'- cAACUGAACAGCGCCCACGCGGAUCAGa -3' miRNA: 3'- -UUGACUUGUCGCGGGUGCGCCUAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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