Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23039 | 3' | -50.1 | NC_005178.1 | + | 225 | 0.66 | 0.862455 |
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Target: 5'- -cCGCUGGAUgguuUAGcGCuCAUucUGUGCCa -3' miRNA: 3'- cuGCGAUCUA----AUC-CGuGUA--ACACGGa -5' |
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| 23039 | 3' | -50.1 | NC_005178.1 | + | 11132 | 0.66 | 0.844186 |
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Target: 5'- gGAUGCgGGAUcAGGCAUuugGUGCUUc -3' miRNA: 3'- -CUGCGaUCUAaUCCGUGuaaCACGGA- -5' |
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| 23039 | 3' | -50.1 | NC_005178.1 | + | 18857 | 0.68 | 0.739124 |
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Target: 5'- uACGCaa---UAGGCACAaUGUGCCg -3' miRNA: 3'- cUGCGaucuaAUCCGUGUaACACGGa -5' |
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| 23039 | 3' | -50.1 | NC_005178.1 | + | 15259 | 0.7 | 0.633315 |
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Target: 5'- gGAUGCagacGGAUUAGGC-CGUUG-GCCa -3' miRNA: 3'- -CUGCGa---UCUAAUCCGuGUAACaCGGa -5' |
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| 23039 | 3' | -50.1 | NC_005178.1 | + | 18816 | 0.74 | 0.407916 |
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Target: 5'- uGCGU---AUUAGGCACAaUGUGCCUa -3' miRNA: 3'- cUGCGaucUAAUCCGUGUaACACGGA- -5' |
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| 23039 | 3' | -50.1 | NC_005178.1 | + | 18770 | 0.75 | 0.35862 |
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Target: 5'- gGGCGUUAGGggaaaUGaauucggacaagcGGCACAUUGUGCCUa -3' miRNA: 3'- -CUGCGAUCUa----AU-------------CCGUGUAACACGGA- -5' |
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| 23039 | 3' | -50.1 | NC_005178.1 | + | 18886 | 1.09 | 0.001785 |
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Target: 5'- uGACGCUAGAUUAGGCACAUUGUGCCUa -3' miRNA: 3'- -CUGCGAUCUAAUCCGUGUAACACGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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