Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23042 | 3' | -57.7 | NC_005178.1 | + | 34923 | 0.67 | 0.412096 |
Target: 5'- cGCCCgaggcguccGGUCGggcGGAAUCCAGcCCGGUg- -3' miRNA: 3'- -CGGGa--------CUAGU---CCUUGGGUC-GGCCAgu -5' |
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23042 | 3' | -57.7 | NC_005178.1 | + | 31435 | 0.66 | 0.45102 |
Target: 5'- uGgCCUGAUauccagcugcucCAGGAACCgcuggCGGCCGGcUCGc -3' miRNA: 3'- -CgGGACUA------------GUCCUUGG-----GUCGGCC-AGU- -5' |
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23042 | 3' | -57.7 | NC_005178.1 | + | 31086 | 0.68 | 0.33257 |
Target: 5'- uGCCCUGG--AGGAacgcACCUGGaCGGUCAg -3' miRNA: 3'- -CGGGACUagUCCU----UGGGUCgGCCAGU- -5' |
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23042 | 3' | -57.7 | NC_005178.1 | + | 25465 | 0.72 | 0.20328 |
Target: 5'- cGCCUUGAUCcGGGccGCCU-GCUGGUCGg -3' miRNA: 3'- -CGGGACUAGuCCU--UGGGuCGGCCAGU- -5' |
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23042 | 3' | -57.7 | NC_005178.1 | + | 24191 | 0.69 | 0.308687 |
Target: 5'- cGCCC--GUCAcGGAGCCCGccGCCuuGGUCAc -3' miRNA: 3'- -CGGGacUAGU-CCUUGGGU--CGG--CCAGU- -5' |
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23042 | 3' | -57.7 | NC_005178.1 | + | 23576 | 1.11 | 0.000255 |
Target: 5'- cGCCCUGAUCAGGAACCCAGCCGGUCAg -3' miRNA: 3'- -CGGGACUAGUCCUUGGGUCGGCCAGU- -5' |
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23042 | 3' | -57.7 | NC_005178.1 | + | 23525 | 0.67 | 0.387026 |
Target: 5'- uUCCUGAUCAGGGcgucacuccgcugGCCCGcagcccgcaggcuucGCCaGUCAg -3' miRNA: 3'- cGGGACUAGUCCU-------------UGGGU---------------CGGcCAGU- -5' |
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23042 | 3' | -57.7 | NC_005178.1 | + | 17725 | 0.67 | 0.421632 |
Target: 5'- aCCCUGGagAcGAccgACCgGGCCGGUCGg -3' miRNA: 3'- cGGGACUagUcCU---UGGgUCGGCCAGU- -5' |
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23042 | 3' | -57.7 | NC_005178.1 | + | 17719 | 0.68 | 0.375319 |
Target: 5'- cGCCCgucucGA-CGGucACCUGGCCGGUCu -3' miRNA: 3'- -CGGGa----CUaGUCcuUGGGUCGGCCAGu -5' |
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23042 | 3' | -57.7 | NC_005178.1 | + | 16596 | 0.72 | 0.20328 |
Target: 5'- cCCCUGGUCaagcuGGGcgUCCAgguGCCGGUCAa -3' miRNA: 3'- cGGGACUAG-----UCCuuGGGU---CGGCCAGU- -5' |
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23042 | 3' | -57.7 | NC_005178.1 | + | 15634 | 0.71 | 0.244965 |
Target: 5'- gGCCUcGAUCAGGAugUgAGCCagcuuggcuggGGUCAg -3' miRNA: 3'- -CGGGaCUAGUCCUugGgUCGG-----------CCAGU- -5' |
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23042 | 3' | -57.7 | NC_005178.1 | + | 3162 | 0.67 | 0.387937 |
Target: 5'- gGCCCUuGGUagaucgucaugaaggCAGGcGCCCAGUCGGg-- -3' miRNA: 3'- -CGGGA-CUA---------------GUCCuUGGGUCGGCCagu -5' |
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23042 | 3' | -57.7 | NC_005178.1 | + | 2894 | 0.68 | 0.375319 |
Target: 5'- aCCCUGAUgCuGGAcauggcgcGCCuCGGCCaGGUCGa -3' miRNA: 3'- cGGGACUA-GuCCU--------UGG-GUCGG-CCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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