Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23046 | 5' | -55.5 | NC_005178.1 | + | 37165 | 0.66 | 0.600962 |
Target: 5'- aGGUCGucGGGAUGGAACg-CCAGGUc -3' miRNA: 3'- gCCAGCc-CCUUGUCUUGaaGGUCCGa -5' |
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23046 | 5' | -55.5 | NC_005178.1 | + | 27270 | 0.66 | 0.568491 |
Target: 5'- uGGUCGaGGGGcguagacggcccuuuCGGGGCUUUCGGGUc -3' miRNA: 3'- gCCAGC-CCCUu--------------GUCUUGAAGGUCCGa -5' |
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23046 | 5' | -55.5 | NC_005178.1 | + | 8908 | 0.67 | 0.524493 |
Target: 5'- gCGGUCGGuGGcguCGGGGCUcacacccggcacgcgCCGGGCg -3' miRNA: 3'- -GCCAGCC-CCuu-GUCUUGAa--------------GGUCCGa -5' |
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23046 | 5' | -55.5 | NC_005178.1 | + | 10330 | 0.68 | 0.460147 |
Target: 5'- cCGGgcgCGGaGGGGCGaAGCgcgCCGGGCUg -3' miRNA: 3'- -GCCa--GCC-CCUUGUcUUGaa-GGUCCGA- -5' |
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23046 | 5' | -55.5 | NC_005178.1 | + | 23166 | 0.7 | 0.338769 |
Target: 5'- uGGgcgcCGGGGugcCGGAGCUggucagggCCAGGCUg -3' miRNA: 3'- gCCa---GCCCCuu-GUCUUGAa-------GGUCCGA- -5' |
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23046 | 5' | -55.5 | NC_005178.1 | + | 21279 | 0.71 | 0.313474 |
Target: 5'- cCGGcCGGGGAGCGGGuuucgguGCcgUCCGuGGCg -3' miRNA: 3'- -GCCaGCCCCUUGUCU-------UGa-AGGU-CCGa -5' |
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23046 | 5' | -55.5 | NC_005178.1 | + | 5416 | 0.75 | 0.181667 |
Target: 5'- uGGUCGGGGGcaagagcccccgccaGCAGAugUgggaaguaaUCCGGGCc -3' miRNA: 3'- gCCAGCCCCU---------------UGUCUugA---------AGGUCCGa -5' |
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23046 | 5' | -55.5 | NC_005178.1 | + | 24729 | 1.09 | 0.000599 |
Target: 5'- aCGGUCGGGGAACAGAACUUCCAGGCUg -3' miRNA: 3'- -GCCAGCCCCUUGUCUUGAAGGUCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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