Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23047 | 3' | -61.5 | NC_005178.1 | + | 8755 | 0.66 | 0.314874 |
Target: 5'- uGCcg-UGGGCGaCUuggccagcguGCGGGCGGCGGuCu -3' miRNA: 3'- -UGauaGCCCGC-GG----------UGUCCGCCGCCuG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 34733 | 0.66 | 0.29243 |
Target: 5'- gGCUGgccaGGGUucagGCUACGGGCgcGGCGGuCg -3' miRNA: 3'- -UGAUag--CCCG----CGGUGUCCG--CCGCCuG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 19888 | 0.66 | 0.290979 |
Target: 5'- ----aUGGGCGCCcuauggaugugcCGGGCuGGCGGAUc -3' miRNA: 3'- ugauaGCCCGCGGu-----------GUCCG-CCGCCUG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 27728 | 0.66 | 0.277475 |
Target: 5'- cGCUGUUGcGCGCCcaugcccACGGcaGCGGCGGcACg -3' miRNA: 3'- -UGAUAGCcCGCGG-------UGUC--CGCCGCC-UG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 32943 | 0.67 | 0.244963 |
Target: 5'- gGCUGguuuacCGGGgGCUACGucgaguGGCaGGUGGACg -3' miRNA: 3'- -UGAUa-----GCCCgCGGUGU------CCG-CCGCCUG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 23362 | 0.67 | 0.238729 |
Target: 5'- -----gGGGCGUCGCaccaGGGCGGCagucGGGCg -3' miRNA: 3'- ugauagCCCGCGGUG----UCCGCCG----CCUG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 3730 | 0.68 | 0.22666 |
Target: 5'- cCUuggCGGcGCGaauCCACAGGCucuGGUGGACg -3' miRNA: 3'- uGAua-GCC-CGC---GGUGUCCG---CCGCCUG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 4106 | 0.68 | 0.217381 |
Target: 5'- aGCUcgCGGGCgaaguagcgguuGCCgaugaacuccaguucGCA-GCGGCGGACg -3' miRNA: 3'- -UGAuaGCCCG------------CGG---------------UGUcCGCCGCCUG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 28307 | 0.68 | 0.215112 |
Target: 5'- aGCUGcCGGG-GCCGCuggaguggcuGGUGGCGGGa -3' miRNA: 3'- -UGAUaGCCCgCGGUGu---------CCGCCGCCUg -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 7432 | 0.68 | 0.204072 |
Target: 5'- aGCUggCGGGCGUCAUcuGCGGCGa-- -3' miRNA: 3'- -UGAuaGCCCGCGGUGucCGCCGCcug -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 22267 | 0.68 | 0.198738 |
Target: 5'- cGCcaggCuGGC-CCGcCAGGCGGCGGGCg -3' miRNA: 3'- -UGaua-GcCCGcGGU-GUCCGCCGCCUG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 29049 | 0.69 | 0.193526 |
Target: 5'- uGCUAUCGGuGcCGCC-CAGGCcaggGGCGcGCa -3' miRNA: 3'- -UGAUAGCC-C-GCGGuGUCCG----CCGCcUG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 3025 | 0.69 | 0.188435 |
Target: 5'- cGCgcUCGGGCgGCCGCgAGGaucGCGGGCc -3' miRNA: 3'- -UGauAGCCCG-CGGUG-UCCgc-CGCCUG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 34916 | 0.69 | 0.183461 |
Target: 5'- gGCguccgGUCGGGCGgaauccagcCCGguGGUGGCGG-Cg -3' miRNA: 3'- -UGa----UAGCCCGC---------GGUguCCGCCGCCuG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 3963 | 0.7 | 0.155997 |
Target: 5'- gGCUucGUUGGGgGUCAUGuGGCGGCGG-Cg -3' miRNA: 3'- -UGA--UAGCCCgCGGUGU-CCGCCGCCuG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 34960 | 0.7 | 0.155997 |
Target: 5'- cGCgauaGGGUGCCucgauCAGGCGGCGcACa -3' miRNA: 3'- -UGauagCCCGCGGu----GUCCGCCGCcUG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 3128 | 0.73 | 0.091958 |
Target: 5'- --aGUCGGGaaCGCuCAugucCGGGCGGCGGACc -3' miRNA: 3'- ugaUAGCCC--GCG-GU----GUCCGCCGCCUG- -5' |
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23047 | 3' | -61.5 | NC_005178.1 | + | 25053 | 1.09 | 0.000144 |
Target: 5'- uACUAUCGGGCGCCACAGGCGGCGGACg -3' miRNA: 3'- -UGAUAGCCCGCGGUGUCCGCCGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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