Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23047 | 5' | -59.1 | NC_005178.1 | + | 15583 | 0.71 | 0.16331 |
Target: 5'- gUGGUUGGCgaaCUCuuugGCCAGACCGGCc- -3' miRNA: 3'- -ACCAACCGac-GGG----UGGUCUGGUCGua -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 17078 | 0.66 | 0.361292 |
Target: 5'- cGGUUGGCguUG-CCAUCAGucgauccgauCCAGCGUg -3' miRNA: 3'- aCCAACCG--ACgGGUGGUCu---------GGUCGUA- -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 36132 | 0.66 | 0.361292 |
Target: 5'- gUGGUcgcacccGUUGCCCACCAGcggGCCAaGCGg -3' miRNA: 3'- -ACCAac-----CGACGGGUGGUC---UGGU-CGUa -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 20458 | 0.66 | 0.343992 |
Target: 5'- aGGUUGGUcGCCUgggcguCCAgGGCCGGCu- -3' miRNA: 3'- aCCAACCGaCGGGu-----GGU-CUGGUCGua -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 29365 | 0.67 | 0.311207 |
Target: 5'- cGGUaGGa-GCCCACCAcGGCgAGCGg -3' miRNA: 3'- aCCAaCCgaCGGGUGGU-CUGgUCGUa -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 12986 | 0.67 | 0.304166 |
Target: 5'- ---cUGGCgcgccacguccuccaGCCCACCAccGGCCAGCAa -3' miRNA: 3'- accaACCGa--------------CGGGUGGU--CUGGUCGUa -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 6548 | 0.68 | 0.27364 |
Target: 5'- cGGUgaccUGGCcgaacugGCUCACCAGcuCCGGCAa -3' miRNA: 3'- aCCA----ACCGa------CGGGUGGUCu-GGUCGUa -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 25557 | 0.68 | 0.246274 |
Target: 5'- cUGG-UGGCUGCCCGCguGG-CGGUGUu -3' miRNA: 3'- -ACCaACCGACGGGUGguCUgGUCGUA- -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 34890 | 0.69 | 0.2153 |
Target: 5'- cGGUggUGGCggcGCCaCGCCGGuugucGCCGGCAg -3' miRNA: 3'- aCCA--ACCGa--CGG-GUGGUC-----UGGUCGUa -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 10422 | 0.71 | 0.145874 |
Target: 5'- -----cGCUGCCCACCAGcucgGCCAGCGc -3' miRNA: 3'- accaacCGACGGGUGGUC----UGGUCGUa -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 9540 | 0.72 | 0.137807 |
Target: 5'- aUGGgUGGCUGCUCAUCAGuACCGGa-- -3' miRNA: 3'- -ACCaACCGACGGGUGGUC-UGGUCgua -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 14758 | 0.77 | 0.053947 |
Target: 5'- aGG--GGCUGCCCAUCcGGCCAGCAg -3' miRNA: 3'- aCCaaCCGACGGGUGGuCUGGUCGUa -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 25017 | 1.05 | 0.00037 |
Target: 5'- cUGGUUGGCUGCCCACCAGACCAGCAUc -3' miRNA: 3'- -ACCAACCGACGGGUGGUCUGGUCGUA- -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 16341 | 0.71 | 0.154369 |
Target: 5'- cUGGgcGGCacGCUCaccaGCCAGACCAGCGa -3' miRNA: 3'- -ACCaaCCGa-CGGG----UGGUCUGGUCGUa -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 21453 | 0.65 | 0.369273 |
Target: 5'- gUGGUUGaGCaccuuuccgaagaUGCgCCGCUGGAUCGGCGc -3' miRNA: 3'- -ACCAAC-CG-------------ACG-GGUGGUCUGGUCGUa -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 22271 | 0.67 | 0.288214 |
Target: 5'- ----aGGCUgGCCCGCCAGG-CGGCGg -3' miRNA: 3'- accaaCCGA-CGGGUGGUCUgGUCGUa -5' |
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23047 | 5' | -59.1 | NC_005178.1 | + | 17998 | 0.71 | 0.158783 |
Target: 5'- ----cGGCUGCCCAgggugcccagcuCCAGGCUGGCGUg -3' miRNA: 3'- accaaCCGACGGGU------------GGUCUGGUCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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