Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23049 | 3' | -57.9 | NC_005178.1 | + | 25936 | 1.07 | 0.000356 |
Target: 5'- gCAACUGCCGCAUGGCCUGGCGGUACUg -3' miRNA: 3'- -GUUGACGGCGUACCGGACCGCCAUGA- -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 22937 | 0.75 | 0.097791 |
Target: 5'- cCAGCUGCCGC-UGGCCcagugccuGUGGUGCUg -3' miRNA: 3'- -GUUGACGGCGuACCGGac------CGCCAUGA- -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 16050 | 0.72 | 0.172933 |
Target: 5'- gGGCUGuuCCGCGugcUGGCCUGGCcGUACc -3' miRNA: 3'- gUUGAC--GGCGU---ACCGGACCGcCAUGa -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 18092 | 0.72 | 0.172933 |
Target: 5'- --cCUGCCGCAgcGCCUGGCccagcccgccauGGUGCUg -3' miRNA: 3'- guuGACGGCGUacCGGACCG------------CCAUGA- -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 3793 | 0.72 | 0.172933 |
Target: 5'- uCAGCUugaaggugGCCaGCuUGGCCUGGCGGUcCa -3' miRNA: 3'- -GUUGA--------CGG-CGuACCGGACCGCCAuGa -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 16266 | 0.72 | 0.187907 |
Target: 5'- -cGCUGCgGCAUGGCCgaGGcCGGUcaccGCUc -3' miRNA: 3'- guUGACGgCGUACCGGa-CC-GCCA----UGA- -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 16657 | 0.7 | 0.233502 |
Target: 5'- -cGCUGCCaGCcaAUGGCgaGGCGGUcCUg -3' miRNA: 3'- guUGACGG-CG--UACCGgaCCGCCAuGA- -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 33901 | 0.69 | 0.280717 |
Target: 5'- --cCUGCCGC---GCCUGGCGGcgaUGCUc -3' miRNA: 3'- guuGACGGCGuacCGGACCGCC---AUGA- -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 12437 | 0.69 | 0.266498 |
Target: 5'- gAGCcaagGCCGCcgguaGUGGCCUGGgCGGcACg -3' miRNA: 3'- gUUGa---CGGCG-----UACCGGACC-GCCaUGa -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 24884 | 0.68 | 0.303166 |
Target: 5'- uGAUUGCCGUgagcuGUGGCaaGGCGGaugACUg -3' miRNA: 3'- gUUGACGGCG-----UACCGgaCCGCCa--UGA- -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 26339 | 0.68 | 0.310949 |
Target: 5'- gAACgGCacucaGUAUgaGGCCgUGGCGGUACUg -3' miRNA: 3'- gUUGaCGg----CGUA--CCGG-ACCGCCAUGA- -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 28306 | 0.68 | 0.310949 |
Target: 5'- gAGCUGCCGgGgccgcuggagUGGCUggUGGCGGgagugGCUg -3' miRNA: 3'- gUUGACGGCgU----------ACCGG--ACCGCCa----UGA- -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 36112 | 0.68 | 0.335206 |
Target: 5'- ---aUGCCGCccagguUGGgCUGGCgGGUGCa -3' miRNA: 3'- guugACGGCGu-----ACCgGACCG-CCAUGa -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 21096 | 0.68 | 0.32697 |
Target: 5'- uCAGCgcgGCCcagGUAggucaGGCCggUGGCGGUGCUg -3' miRNA: 3'- -GUUGa--CGG---CGUa----CCGG--ACCGCCAUGA- -5' |
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23049 | 3' | -57.9 | NC_005178.1 | + | 31508 | 0.66 | 0.435501 |
Target: 5'- uCGACUuCCGCGacggcGGUCUGGCGaUGCUg -3' miRNA: 3'- -GUUGAcGGCGUa----CCGGACCGCcAUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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