Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23049 | 5' | -58.2 | NC_005178.1 | + | 31915 | 0.67 | 0.360347 |
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Target: 5'- ---cUGCCGGUCAuCGGCCAUcgcugaucGGCa-- -3' miRNA: 3'- cuuuACGGCCGGU-GCCGGUA--------CCGcag -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 32295 | 0.67 | 0.377184 |
|
Target: 5'- ----gGUCGGCUcgaccagGCGGCCcagggcGGCGUCg -3' miRNA: 3'- cuuuaCGGCCGG-------UGCCGGua----CCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 33617 | 0.68 | 0.326647 |
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Target: 5'- -cGAUGCCGuccuggagaaaaGCCAUccaauuuaGGCCAUGGCGc- -3' miRNA: 3'- cuUUACGGC------------CGGUG--------CCGGUACCGCag -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 34738 | 0.66 | 0.415274 |
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Target: 5'- gGAAcgGCUGGCCAggguucaGGCUAcgGGCG-Cg -3' miRNA: 3'- -CUUuaCGGCCGGUg------CCGGUa-CCGCaG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 35701 | 0.66 | 0.405767 |
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Target: 5'- ----cGCUGGCCGaGGaCAUGGCGg- -3' miRNA: 3'- cuuuaCGGCCGGUgCCgGUACCGCag -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 35866 | 0.72 | 0.16379 |
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Target: 5'- ----aGCCGGaaaCCGaGGCCGUGGCGUUc -3' miRNA: 3'- cuuuaCGGCC---GGUgCCGGUACCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 37048 | 0.67 | 0.38717 |
|
Target: 5'- aGAGuggGCCGGCUcgcuggacgagGCGGCagcacucgcUGGCGUCc -3' miRNA: 3'- -CUUua-CGGCCGG-----------UGCCGgu-------ACCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 37262 | 0.67 | 0.351699 |
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Target: 5'- --uGUGCCGGUa---GCgAUGGCGUCa -3' miRNA: 3'- cuuUACGGCCGgugcCGgUACCGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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