Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23052 | 3' | -53 | NC_005178.1 | + | 29428 | 0.66 | 0.761735 |
Target: 5'- aGGGGCUgcugaugccccggUGCCGGUCGcuaACGucGGa -3' miRNA: 3'- gCUCCGGa------------ACGGUCAGCag-UGUuuCC- -5' |
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23052 | 3' | -53 | NC_005178.1 | + | 33578 | 0.66 | 0.73802 |
Target: 5'- uCGAGGCCgacgUGCUGGUCGcgaccggcgacgcgcUCGCGc--- -3' miRNA: 3'- -GCUCCGGa---ACGGUCAGC---------------AGUGUuucc -5' |
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23052 | 3' | -53 | NC_005178.1 | + | 16256 | 0.66 | 0.722629 |
Target: 5'- --uGGCCgagGCCGGUCaccgcUCGCAGcAGGg -3' miRNA: 3'- gcuCCGGaa-CGGUCAGc----AGUGUU-UCC- -5' |
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23052 | 3' | -53 | NC_005178.1 | + | 22464 | 0.67 | 0.70032 |
Target: 5'- gCGAGuuCUUGCCGGacgaCGUCggcgGCGAGGGu -3' miRNA: 3'- -GCUCcgGAACGGUCa---GCAG----UGUUUCC- -5' |
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23052 | 3' | -53 | NC_005178.1 | + | 17790 | 0.67 | 0.677725 |
Target: 5'- cCGAccGGCCcgGUCGGUCGUCuCcAGGGu -3' miRNA: 3'- -GCU--CCGGaaCGGUCAGCAGuGuUUCC- -5' |
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23052 | 3' | -53 | NC_005178.1 | + | 11615 | 0.67 | 0.666351 |
Target: 5'- --uGGCCcUGCCAGUUGUUGCGu--- -3' miRNA: 3'- gcuCCGGaACGGUCAGCAGUGUuucc -5' |
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23052 | 3' | -53 | NC_005178.1 | + | 3163 | 0.67 | 0.666351 |
Target: 5'- --cGGcCCUUGgUAGaUCGUCAUGAAGGc -3' miRNA: 3'- gcuCC-GGAACgGUC-AGCAGUGUUUCC- -5' |
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23052 | 3' | -53 | NC_005178.1 | + | 31548 | 0.69 | 0.575067 |
Target: 5'- uCGAGGCCg-GCCGuGUCG-CACGGAa- -3' miRNA: 3'- -GCUCCGGaaCGGU-CAGCaGUGUUUcc -5' |
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23052 | 3' | -53 | NC_005178.1 | + | 26560 | 0.7 | 0.530311 |
Target: 5'- gCGAGGCUUUGaCCGGuUCGUCGgcCAGcuuGGa -3' miRNA: 3'- -GCUCCGGAAC-GGUC-AGCAGU--GUUu--CC- -5' |
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23052 | 3' | -53 | NC_005178.1 | + | 9408 | 0.71 | 0.455456 |
Target: 5'- gCGAGcgcGCCgUGCCAGgcacUCGUCGCAGgcgcAGGa -3' miRNA: 3'- -GCUC---CGGaACGGUC----AGCAGUGUU----UCC- -5' |
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23052 | 3' | -53 | NC_005178.1 | + | 26509 | 0.72 | 0.405628 |
Target: 5'- cCGAaacaGCCUUGCCGGUCGcuuccugcaUCGCGAuGGa -3' miRNA: 3'- -GCUc---CGGAACGGUCAGC---------AGUGUUuCC- -5' |
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23052 | 3' | -53 | NC_005178.1 | + | 28075 | 1.11 | 0.000857 |
Target: 5'- gCGAGGCCUUGCCAGUCGUCACAAAGGu -3' miRNA: 3'- -GCUCCGGAACGGUCAGCAGUGUUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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