miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23053 3' -50.9 NC_005178.1 + 26495 0.66 0.820728
Target:  5'- cCGGUCgCUUCCUGcAUcGCGaUGGAGgUa -3'
miRNA:   3'- aGUCAG-GAAGGAC-UAaCGC-AUCUCgG- -5'
23053 3' -50.9 NC_005178.1 + 17064 0.67 0.800716
Target:  5'- aUCAGUCgaUCC-GAUccaGCGUGccguuGAGCCg -3'
miRNA:   3'- -AGUCAGgaAGGaCUAa--CGCAU-----CUCGG- -5'
23053 3' -50.9 NC_005178.1 + 7771 0.68 0.736329
Target:  5'- -gAGUCCagUCCUGGU-GCG-AG-GCCa -3'
miRNA:   3'- agUCAGGa-AGGACUAaCGCaUCuCGG- -5'
23053 3' -50.9 NC_005178.1 + 29805 1.14 0.000738
Target:  5'- gUCAGUCCUUCCUGAUUGCGUAGAGCCa -3'
miRNA:   3'- -AGUCAGGAAGGACUAACGCAUCUCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.