Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23053 | 3' | -50.9 | NC_005178.1 | + | 26495 | 0.66 | 0.820728 |
Target: 5'- cCGGUCgCUUCCUGcAUcGCGaUGGAGgUa -3' miRNA: 3'- aGUCAG-GAAGGAC-UAaCGC-AUCUCgG- -5' |
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23053 | 3' | -50.9 | NC_005178.1 | + | 17064 | 0.67 | 0.800716 |
Target: 5'- aUCAGUCgaUCC-GAUccaGCGUGccguuGAGCCg -3' miRNA: 3'- -AGUCAGgaAGGaCUAa--CGCAU-----CUCGG- -5' |
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23053 | 3' | -50.9 | NC_005178.1 | + | 7771 | 0.68 | 0.736329 |
Target: 5'- -gAGUCCagUCCUGGU-GCG-AG-GCCa -3' miRNA: 3'- agUCAGGa-AGGACUAaCGCaUCuCGG- -5' |
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23053 | 3' | -50.9 | NC_005178.1 | + | 29805 | 1.14 | 0.000738 |
Target: 5'- gUCAGUCCUUCCUGAUUGCGUAGAGCCa -3' miRNA: 3'- -AGUCAGGAAGGACUAACGCAUCUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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