Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23054 | 5' | -57.7 | NC_005178.1 | + | 30437 | 1.09 | 0.000414 |
Target: 5'- cUGUAGUUUCCGCGACCGGGGCCGACGa -3' miRNA: 3'- -ACAUCAAAGGCGCUGGCCCCGGCUGC- -5' |
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23054 | 5' | -57.7 | NC_005178.1 | + | 17313 | 0.72 | 0.211467 |
Target: 5'- aGUGGUUgcgCCG-GACggCGGGGCCGAgGu -3' miRNA: 3'- aCAUCAAa--GGCgCUG--GCCCCGGCUgC- -5' |
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23054 | 5' | -57.7 | NC_005178.1 | + | 12647 | 0.7 | 0.31093 |
Target: 5'- cUGUAcGgcUCgGCGGCCGGcGcGCUGGCGg -3' miRNA: 3'- -ACAU-CaaAGgCGCUGGCC-C-CGGCUGC- -5' |
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23054 | 5' | -57.7 | NC_005178.1 | + | 35846 | 0.69 | 0.351185 |
Target: 5'- aGgcGUUUCCGCaugagccuGACgaUGGGGCCGAa- -3' miRNA: 3'- aCauCAAAGGCG--------CUG--GCCCCGGCUgc -5' |
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23054 | 5' | -57.7 | NC_005178.1 | + | 3445 | 0.68 | 0.404182 |
Target: 5'- gGUGGUgaUUUC-CGGCCGgaaGGGCCGGCc -3' miRNA: 3'- aCAUCA--AAGGcGCUGGC---CCCGGCUGc -5' |
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23054 | 5' | -57.7 | NC_005178.1 | + | 30801 | 0.66 | 0.482166 |
Target: 5'- --cGGUcgcggCCuCGACCuGGGCCGGCGu -3' miRNA: 3'- acaUCAaa---GGcGCUGGcCCCGGCUGC- -5' |
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23054 | 5' | -57.7 | NC_005178.1 | + | 4068 | 0.66 | 0.492429 |
Target: 5'- cUGgAGgaaUUCCacgGCGACCaGGuGGCCGugGg -3' miRNA: 3'- -ACaUCa--AAGG---CGCUGG-CC-CCGGCugC- -5' |
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23054 | 5' | -57.7 | NC_005178.1 | + | 16965 | 0.66 | 0.5122 |
Target: 5'- cGUuacugUUCGUGGCCgacaccuGGGGCCGGCu -3' miRNA: 3'- aCAucaa-AGGCGCUGG-------CCCCGGCUGc -5' |
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23054 | 5' | -57.7 | NC_005178.1 | + | 28131 | 0.66 | 0.523794 |
Target: 5'- gGUGGcaccgauggCgGCGcCCaGGGGCUGACGg -3' miRNA: 3'- aCAUCaaa------GgCGCuGG-CCCCGGCUGC- -5' |
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23054 | 5' | -57.7 | NC_005178.1 | + | 12467 | 0.66 | 0.472007 |
Target: 5'- --cAGUcaCCGCcACCGGGGUCGggcACGg -3' miRNA: 3'- acaUCAaaGGCGcUGGCCCCGGC---UGC- -5' |
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23054 | 5' | -57.7 | NC_005178.1 | + | 22614 | 0.66 | 0.482166 |
Target: 5'- gGUGGccaUCCGUGGCCaGGGGCgGuCc -3' miRNA: 3'- aCAUCaa-AGGCGCUGG-CCCCGgCuGc -5' |
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23054 | 5' | -57.7 | NC_005178.1 | + | 24397 | 0.66 | 0.513249 |
Target: 5'- -aUAGg--CCGCuuugaacggcugGGUCGGGGCCGGCGu -3' miRNA: 3'- acAUCaaaGGCG------------CUGGCCCCGGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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