Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23055 | 3' | -53.5 | NC_005178.1 | + | 31859 | 0.66 | 0.689457 |
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Target: 5'- cCACCuUGGAcaacGGCAgcgcgauuccccgcGUGACCGACgucgCCGGGa -3' miRNA: 3'- -GUGG-GCCU----UCGU--------------UAUUGGCUG----GGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 22066 | 0.73 | 0.329543 |
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Target: 5'- aGCCCaGGAAGCA---GCCGGaauucguuuCCCAGGu -3' miRNA: 3'- gUGGG-CCUUCGUuauUGGCU---------GGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 26910 | 0.67 | 0.681581 |
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Target: 5'- aGCgCgGGAAGCAAUucACCGAUgcccuUCAGGa -3' miRNA: 3'- gUG-GgCCUUCGUUAu-UGGCUG-----GGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 22521 | 0.67 | 0.670286 |
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Target: 5'- aCACCCaGGAauaacccaccccAGCAGaggGACCGcCCCuGGc -3' miRNA: 3'- -GUGGG-CCU------------UCGUUa--UUGGCuGGGuCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 16724 | 0.67 | 0.670286 |
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Target: 5'- gCGCCCuGGuccucGCAucgcugcGCUGGCCCAGGu -3' miRNA: 3'- -GUGGG-CCuu---CGUuau----UGGCUGGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 17909 | 0.67 | 0.658953 |
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Target: 5'- gCGCCUGGcAGGCGuuugugGACCGcuccacgucgcCCCAGGg -3' miRNA: 3'- -GUGGGCC-UUCGUua----UUGGCu----------GGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 9660 | 0.67 | 0.63622 |
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Target: 5'- gCGgCCGGuGGCuucgccgAGCCGGCCgAGGc -3' miRNA: 3'- -GUgGGCCuUCGuua----UUGGCUGGgUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 7965 | 0.68 | 0.621428 |
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Target: 5'- aACaaGGAAGCGAUccucaagaacccgaGGCCG-UCCAGGg -3' miRNA: 3'- gUGggCCUUCGUUA--------------UUGGCuGGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 17843 | 0.68 | 0.602115 |
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Target: 5'- gCACCCuGGGAGCGccuGCCG-CCCucGGc -3' miRNA: 3'- -GUGGG-CCUUCGUuauUGGCuGGGu-CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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