Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23057 | 5' | -58.9 | NC_005178.1 | + | 17296 | 0.66 | 0.379225 |
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Target: 5'- --uGGUGggGGCGUCagauagUgGuuGCGCc -3' miRNA: 3'- uauCCACuuCCGCAGg-----AgCggCGCGu -5' |
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| 23057 | 5' | -58.9 | NC_005178.1 | + | 20597 | 0.66 | 0.361525 |
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Target: 5'- cUGGGUGAcagcuucaacGGGCGcCCacuUCGCCGgGUc -3' miRNA: 3'- uAUCCACU----------UCCGCaGG---AGCGGCgCGu -5' |
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| 23057 | 5' | -58.9 | NC_005178.1 | + | 34967 | 0.67 | 0.327882 |
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Target: 5'- ---cGUGAGcGCGUCCUacuugGCCGCGCu -3' miRNA: 3'- uaucCACUUcCGCAGGAg----CGGCGCGu -5' |
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| 23057 | 5' | -58.9 | NC_005178.1 | + | 21168 | 0.67 | 0.319841 |
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Target: 5'- ----uUGAAGGCGUUCagGCgGCGCAc -3' miRNA: 3'- uauccACUUCCGCAGGagCGgCGCGU- -5' |
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| 23057 | 5' | -58.9 | NC_005178.1 | + | 28544 | 0.69 | 0.260847 |
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Target: 5'- cUGGGcGggGGcCGUCC--GCCGCGCc -3' miRNA: 3'- uAUCCaCuuCC-GCAGGagCGGCGCGu -5' |
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| 23057 | 5' | -58.9 | NC_005178.1 | + | 6483 | 0.7 | 0.211595 |
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Target: 5'- -cGGGUGuguuuugucaucgcGGCGcgCCUCGCCGCGaCAa -3' miRNA: 3'- uaUCCACuu------------CCGCa-GGAGCGGCGC-GU- -5' |
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| 23057 | 5' | -58.9 | NC_005178.1 | + | 10931 | 0.73 | 0.139512 |
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Target: 5'- -----cGAAGGCGUCCUCGaCGUGCGc -3' miRNA: 3'- uauccaCUUCCGCAGGAGCgGCGCGU- -5' |
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| 23057 | 5' | -58.9 | NC_005178.1 | + | 33880 | 0.76 | 0.071974 |
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Target: 5'- --cGGUGAagaggaccaGGGCGUCCU-GCCGCGCc -3' miRNA: 3'- uauCCACU---------UCCGCAGGAgCGGCGCGu -5' |
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| 23057 | 5' | -58.9 | NC_005178.1 | + | 31601 | 1.06 | 0.000355 |
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Target: 5'- cAUAGGUGAAGGCGUCCUCGCCGCGCAg -3' miRNA: 3'- -UAUCCACUUCCGCAGGAGCGGCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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