Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23058 | 5' | -54.9 | NC_005178.1 | + | 26629 | 0.66 | 0.632605 |
Target: 5'- cGCCuauagCCAGGcaaUGGACgAGCGGGCg -3' miRNA: 3'- -CGGcaacaGGUUCc--ACCUG-UCGUCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 36630 | 0.66 | 0.625797 |
Target: 5'- uGCUGUca-CCAGGGcGGAUagaacuccaccccguAGCGGGCa -3' miRNA: 3'- -CGGCAacaGGUUCCaCCUG---------------UCGUCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 22560 | 0.66 | 0.598611 |
Target: 5'- uGUCGUUGUCCGAcGcGGACGGCu--- -3' miRNA: 3'- -CGGCAACAGGUUcCaCCUGUCGuccg -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 6527 | 0.67 | 0.576082 |
Target: 5'- uGCgGUUGgCCAAGGgaaugccucUGGA--GCAGGCc -3' miRNA: 3'- -CGgCAACaGGUUCC---------ACCUguCGUCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 9035 | 0.67 | 0.574961 |
Target: 5'- cGCCGUagcucUGUCCGGGGUgauugaagggguuGGACuGCu-GCa -3' miRNA: 3'- -CGGCA-----ACAGGUUCCA-------------CCUGuCGucCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 23439 | 0.67 | 0.564887 |
Target: 5'- cGCCGacGcCCAGGGc-GACGGCcaGGGCg -3' miRNA: 3'- -CGGCaaCaGGUUCCacCUGUCG--UCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 22260 | 0.67 | 0.55375 |
Target: 5'- aGCCGaccgCCAGGcUGGcccgccagGCGGCGGGCg -3' miRNA: 3'- -CGGCaacaGGUUCcACC--------UGUCGUCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 14069 | 0.67 | 0.542679 |
Target: 5'- uGCUGgccGUCC-GGGUGGuccacCAGCucGGGCa -3' miRNA: 3'- -CGGCaa-CAGGuUCCACCu----GUCG--UCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 32865 | 0.67 | 0.542679 |
Target: 5'- uGCCGUg--C--AGGUGcGGCAGUAGGUg -3' miRNA: 3'- -CGGCAacaGguUCCAC-CUGUCGUCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 17936 | 0.67 | 0.536071 |
Target: 5'- aCCGg-GUCCAGGagcacGUccuggaccucgccgaGGGCGGCAGGCg -3' miRNA: 3'- cGGCaaCAGGUUC-----CA---------------CCUGUCGUCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 34379 | 0.68 | 0.509939 |
Target: 5'- uGCCGcUGUUCGAcGaGGACAGC-GGCc -3' miRNA: 3'- -CGGCaACAGGUUcCaCCUGUCGuCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 10189 | 0.68 | 0.488577 |
Target: 5'- gGCCag-GUCUGAGGUGGAucuuCGGgAGGUc -3' miRNA: 3'- -CGGcaaCAGGUUCCACCU----GUCgUCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 14588 | 0.68 | 0.488577 |
Target: 5'- cGCCGcu-UCCAcaGUGGGuuuuuCAGCAGGCg -3' miRNA: 3'- -CGGCaacAGGUucCACCU-----GUCGUCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 30954 | 0.69 | 0.467645 |
Target: 5'- aGCCgGUUGuaauaguugucuUCCAGggcgcucuGGUGGACGGCuGGGUa -3' miRNA: 3'- -CGG-CAAC------------AGGUU--------CCACCUGUCG-UCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 28234 | 0.69 | 0.447184 |
Target: 5'- cGCCGg---CCGAGGcGGAuaCAGCcgAGGCg -3' miRNA: 3'- -CGGCaacaGGUUCCaCCU--GUCG--UCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 26705 | 0.71 | 0.353006 |
Target: 5'- aCCGUUG-CCGGGGUGGcCAa-GGGCg -3' miRNA: 3'- cGGCAACaGGUUCCACCuGUcgUCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 11292 | 0.72 | 0.288379 |
Target: 5'- aGCUGUUcgaGUCCGcccAGGUGGugGcCGGGCu -3' miRNA: 3'- -CGGCAA---CAGGU---UCCACCugUcGUCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 25369 | 0.72 | 0.280988 |
Target: 5'- gGCCGggagcUGUCCGguuuGGG-GGAgacagccgacCAGCAGGCg -3' miRNA: 3'- -CGGCa----ACAGGU----UCCaCCU----------GUCGUCCG- -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 11145 | 0.73 | 0.246279 |
Target: 5'- uGCCGg---CCGAGGUGGAU-GCGGGa -3' miRNA: 3'- -CGGCaacaGGUUCCACCUGuCGUCCg -5' |
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23058 | 5' | -54.9 | NC_005178.1 | + | 3581 | 0.75 | 0.182404 |
Target: 5'- aGCCGUUGUCCAcguagaagauGGccgGGACGccgcccuugauGCAGGCg -3' miRNA: 3'- -CGGCAACAGGUu---------CCa--CCUGU-----------CGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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