Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23059 | 3' | -56.8 | NC_005178.1 | + | 858 | 0.66 | 0.537527 |
Target: 5'- aGGUCGGaaCGCgCAUcgcggaGGUGGCUaggCUGCu -3' miRNA: 3'- cCCAGCC--GUGgGUA------UCGCCGAa--GACG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 4516 | 0.69 | 0.35127 |
Target: 5'- cGGUCGGCagggugaagACCUgggcgGUGGUGGCUUgggugguggCUGCg -3' miRNA: 3'- cCCAGCCG---------UGGG-----UAUCGCCGAA---------GACG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 5963 | 0.67 | 0.453729 |
Target: 5'- cGGGUCGuaGaCC-UGGCGGCgcgUCUcGCg -3' miRNA: 3'- -CCCAGCcgUgGGuAUCGCCGa--AGA-CG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 7119 | 0.67 | 0.453729 |
Target: 5'- aGGGcCGGgCACUCAauGCGGCU-CaGCg -3' miRNA: 3'- -CCCaGCC-GUGGGUauCGCCGAaGaCG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 12413 | 0.68 | 0.39577 |
Target: 5'- uGGG-CGGCACgUugacgGGCGGUUUCgaGCu -3' miRNA: 3'- -CCCaGCCGUGgGua---UCGCCGAAGa-CG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 16637 | 0.66 | 0.537527 |
Target: 5'- aGGGgCGGUAaugacguugCCAUGGCGGCcaggUCgGCc -3' miRNA: 3'- -CCCaGCCGUg--------GGUAUCGCCGa---AGaCG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 17780 | 0.66 | 0.515997 |
Target: 5'- cGGUCGGUcgucUCCAgGGUGGUcaaggUCUGCu -3' miRNA: 3'- cCCAGCCGu---GGGUaUCGCCGa----AGACG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 20041 | 0.68 | 0.39577 |
Target: 5'- aGGGUCuGGUGCUCGUAGUccaggacgGGCggagUCUuGCg -3' miRNA: 3'- -CCCAG-CCGUGGGUAUCG--------CCGa---AGA-CG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 20550 | 0.73 | 0.188263 |
Target: 5'- cGGGggCGGCgACCUucagcugggcgGUGGCGGCggcgaUCUGCu -3' miRNA: 3'- -CCCa-GCCG-UGGG-----------UAUCGCCGa----AGACG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 21267 | 0.73 | 0.193474 |
Target: 5'- cGGGuuUCGGUGCcguCCGUGGCGGCgacggUCUGg -3' miRNA: 3'- -CCC--AGCCGUG---GGUAUCGCCGa----AGACg -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 21465 | 0.7 | 0.287726 |
Target: 5'- --cUCGGCACuuCCAUGGCGauCUUCUGCc -3' miRNA: 3'- cccAGCCGUG--GGUAUCGCc-GAAGACG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 26652 | 0.69 | 0.381139 |
Target: 5'- uGGG-CGGC-CCCAgcgaaUcccuugcugaucgagGGCGGCUaUCUGCu -3' miRNA: 3'- -CCCaGCCGuGGGU-----A---------------UCGCCGA-AGACG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 28126 | 0.72 | 0.23358 |
Target: 5'- uGGGcggUGGCACCgAUGGCGGCgcccaggggCUGa -3' miRNA: 3'- -CCCa--GCCGUGGgUAUCGCCGaa-------GACg -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 28162 | 0.67 | 0.484385 |
Target: 5'- cGGUCGGCGCCU----CGGCUguaUCcGCc -3' miRNA: 3'- cCCAGCCGUGGGuaucGCCGA---AGaCG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 28189 | 0.67 | 0.474053 |
Target: 5'- cGGcCGGCGCCC-UGGCGacuGCUg--GCg -3' miRNA: 3'- cCCaGCCGUGGGuAUCGC---CGAagaCG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 28464 | 0.68 | 0.386583 |
Target: 5'- cGGUCGGCAUCCGccaugcAGCcagGGCUUacuccgCUGCu -3' miRNA: 3'- cCCAGCCGUGGGUa-----UCG---CCGAA------GACG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 29897 | 0.68 | 0.41456 |
Target: 5'- cGGUCGGUgACCCAgccaAG-GGUUUCgGCg -3' miRNA: 3'- cCCAGCCG-UGGGUa---UCgCCGAAGaCG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 32063 | 1.13 | 0.000216 |
Target: 5'- uGGGUCGGCACCCAUAGCGGCUUCUGCu -3' miRNA: 3'- -CCCAGCCGUGGGUAUCGCCGAAGACG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 32296 | 0.74 | 0.173366 |
Target: 5'- cGGUCGGCucgaccaggcgGCCCAgGGCGGCgUCgaugGCc -3' miRNA: 3'- cCCAGCCG-----------UGGGUaUCGCCGaAGa---CG- -5' |
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23059 | 3' | -56.8 | NC_005178.1 | + | 33040 | 0.66 | 0.533195 |
Target: 5'- uGGG-CGGCACCgAgggcauuccggcaGGUGGCcaaCUGCg -3' miRNA: 3'- -CCCaGCCGUGGgUa------------UCGCCGaa-GACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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