Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23060 | 5' | -53.3 | NC_005178.1 | + | 1495 | 0.66 | 0.713525 |
Target: 5'- aGGUAUaGCGCGgG-CCGGCuGCGGa- -3' miRNA: 3'- gCUAUAaCGCGUgUaGGUCG-CGCCag -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 10105 | 0.67 | 0.633489 |
Target: 5'- gCGAUGg-GCGCugG-CCaggAGCGCGGUg -3' miRNA: 3'- -GCUAUaaCGCGugUaGG---UCGCGCCAg -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 11080 | 0.67 | 0.61043 |
Target: 5'- uGAUAggUGCGCAUugccuaccUCCGGgGCGGa- -3' miRNA: 3'- gCUAUa-ACGCGUGu-------AGGUCgCGCCag -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 12552 | 0.66 | 0.713525 |
Target: 5'- aCGGUGUcccaGUGCA-GUCCGGCGCagaggccguauuGGUCg -3' miRNA: 3'- -GCUAUAa---CGCGUgUAGGUCGCG------------CCAG- -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 14713 | 0.66 | 0.711277 |
Target: 5'- gCGGcg--GCGCGCuccugguagaacUCCAGCGCGG-Cg -3' miRNA: 3'- -GCUauaaCGCGUGu-----------AGGUCGCGCCaG- -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 20954 | 0.76 | 0.194205 |
Target: 5'- gGGUGUUGCGguCGguggUCAGgGCGGUCa -3' miRNA: 3'- gCUAUAACGCguGUa---GGUCgCGCCAG- -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 23093 | 0.66 | 0.702253 |
Target: 5'- ----cUUGCGCucgGCAUCCAGgauCGCGGg- -3' miRNA: 3'- gcuauAACGCG---UGUAGGUC---GCGCCag -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 24911 | 0.72 | 0.331694 |
Target: 5'- aCGAgcuuccagUGCuccgucaGCACGUCCAGCGCaauGGUCg -3' miRNA: 3'- -GCUaua-----ACG-------CGUGUAGGUCGCG---CCAG- -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 31361 | 0.67 | 0.656543 |
Target: 5'- ------gGCGCAUAgcCCGGCGCugGGUCa -3' miRNA: 3'- gcuauaaCGCGUGUa-GGUCGCG--CCAG- -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 32132 | 1.11 | 0.000653 |
Target: 5'- cCGAUAUUGCGCACAUCCAGCGCGGUCg -3' miRNA: 3'- -GCUAUAACGCGUGUAGGUCGCGCCAG- -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 32257 | 0.68 | 0.57488 |
Target: 5'- cCGAUGUaGCGCGCAUCUguuucauGGCGCu--- -3' miRNA: 3'- -GCUAUAaCGCGUGUAGG-------UCGCGccag -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 35047 | 0.66 | 0.690906 |
Target: 5'- uGGUcg-GCGCcuCggCCAGCGCGGcCa -3' miRNA: 3'- gCUAuaaCGCGu-GuaGGUCGCGCCaG- -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 35494 | 0.66 | 0.724708 |
Target: 5'- aCGGUAgcGCGUAC--CCGGCGaaGGUCu -3' miRNA: 3'- -GCUAUaaCGCGUGuaGGUCGCg-CCAG- -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 35934 | 0.68 | 0.597777 |
Target: 5'- cCGcAUGUUGCGCugGgaugacuUCCuGCGCcaaccGGUCg -3' miRNA: 3'- -GC-UAUAACGCGugU-------AGGuCGCG-----CCAG- -5' |
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23060 | 5' | -53.3 | NC_005178.1 | + | 36306 | 0.68 | 0.557839 |
Target: 5'- cCGuUGUUGCGCGCGUaguugcuaccgucgcUCGGCGCG-UCg -3' miRNA: 3'- -GCuAUAACGCGUGUA---------------GGUCGCGCcAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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