Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23062 | 5' | -51.9 | NC_005178.1 | + | 34470 | 0.66 | 0.751966 |
Target: 5'- cCGGUCUucuCCUGagugauuUCCAGGAGGCCGCUg -3' miRNA: 3'- -GUUAGAcc-GGAU-------GGGUUCUUUGGCGA- -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 33256 | 0.66 | 0.742001 |
Target: 5'- gGGUCcaUGGCCUACCuCAccAGAccuGAUCGCc -3' miRNA: 3'- gUUAG--ACCGGAUGG-GU--UCU---UUGGCGa -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 10798 | 0.66 | 0.785369 |
Target: 5'- ---cCUGGCCgaGCCCA----GCCGCg -3' miRNA: 3'- guuaGACCGGa-UGGGUucuuUGGCGa -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 20229 | 0.67 | 0.685065 |
Target: 5'- aCGGUCUGgaGCCUcucgcACgCCGAGcgGCCGCg -3' miRNA: 3'- -GUUAGAC--CGGA-----UG-GGUUCuuUGGCGa -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 23282 | 0.67 | 0.73081 |
Target: 5'- cCGAUCUGGCC-ACCCuuGAcgAACaGCg -3' miRNA: 3'- -GUUAGACCGGaUGGGuuCU--UUGgCGa -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 32729 | 0.68 | 0.638398 |
Target: 5'- ---aCUGGCCgACCguGGAuagcuGCCGCa -3' miRNA: 3'- guuaGACCGGaUGGguUCUu----UGGCGa -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 17330 | 0.68 | 0.626684 |
Target: 5'- --cUUUGGCCU--CCAGGAucGGCCGCa -3' miRNA: 3'- guuAGACCGGAugGGUUCU--UUGGCGa -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 28432 | 0.69 | 0.568419 |
Target: 5'- ---cCUGGCCgccACCCGcGAAGCCGa- -3' miRNA: 3'- guuaGACCGGa--UGGGUuCUUUGGCga -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 31976 | 0.69 | 0.590456 |
Target: 5'- ---cCUGGUCUAUgCAcugcgcggccagcAGAAGCCGCUa -3' miRNA: 3'- guuaGACCGGAUGgGU-------------UCUUUGGCGA- -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 18264 | 0.69 | 0.59162 |
Target: 5'- -cAUUUGGCCcgGCCCAuAGAcgccACCGCg -3' miRNA: 3'- guUAGACCGGa-UGGGU-UCUu---UGGCGa -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 28950 | 0.69 | 0.579995 |
Target: 5'- -----aGGCCUGCCCA----GCCGCg -3' miRNA: 3'- guuagaCCGGAUGGGUucuuUGGCGa -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 34796 | 0.69 | 0.576517 |
Target: 5'- uCGAUCcGGCCgACCCGaacgacgguuucagGGAuGCCGCg -3' miRNA: 3'- -GUUAGaCCGGaUGGGU--------------UCUuUGGCGa -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 26677 | 0.7 | 0.545453 |
Target: 5'- ---cCUGGgC-ACCCuGGAAACCGCUu -3' miRNA: 3'- guuaGACCgGaUGGGuUCUUUGGCGA- -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 11819 | 0.7 | 0.511599 |
Target: 5'- ---cCUGGCC-GCCCuGGuGACCGCg -3' miRNA: 3'- guuaGACCGGaUGGGuUCuUUGGCGa -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 4529 | 0.73 | 0.385825 |
Target: 5'- --cUCcGGCCUGCCCAucgagccGGAcGCCGCc -3' miRNA: 3'- guuAGaCCGGAUGGGU-------UCUuUGGCGa -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 31429 | 0.83 | 0.089144 |
Target: 5'- cCGAUCUGGCCUgauAUCCAgcugcuccAGGAACCGCUg -3' miRNA: 3'- -GUUAGACCGGA---UGGGU--------UCUUUGGCGA- -5' |
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23062 | 5' | -51.9 | NC_005178.1 | + | 32756 | 1.09 | 0.001268 |
Target: 5'- cCAAUCUGGCCUACCCAAGAAACCGCUg -3' miRNA: 3'- -GUUAGACCGGAUGGGUUCUUUGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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