Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23063 | 3' | -56.9 | NC_005178.1 | + | 28205 | 0.7 | 0.319841 |
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Target: 5'- cCGAcCGUcAGCCCCUGGgc-GCC-GCCAu -3' miRNA: 3'- -GCU-GCA-UCGGGGGCCauuUGGuCGGU- -5' |
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| 23063 | 3' | -56.9 | NC_005178.1 | + | 28622 | 0.7 | 0.311949 |
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Target: 5'- cCGGCGacGCCCCCGGU-GGCgCGGCgGa -3' miRNA: 3'- -GCUGCauCGGGGGCCAuUUG-GUCGgU- -5' |
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| 23063 | 3' | -56.9 | NC_005178.1 | + | 33998 | 0.7 | 0.304206 |
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Target: 5'- aCGugGUAaCgCCCCGGaAAGCCGGCa- -3' miRNA: 3'- -GCugCAUcG-GGGGCCaUUUGGUCGgu -5' |
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| 23063 | 3' | -56.9 | NC_005178.1 | + | 34572 | 0.7 | 0.296612 |
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Target: 5'- gGGCGU-GCCUaCCGGgGAGCUGGCCGg -3' miRNA: 3'- gCUGCAuCGGG-GGCCaUUUGGUCGGU- -5' |
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| 23063 | 3' | -56.9 | NC_005178.1 | + | 25260 | 0.71 | 0.244966 |
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Target: 5'- aGGCGgcaaucgcuacagAGCCCCCGGcgAAGCCAugguugaccGCCAu -3' miRNA: 3'- gCUGCa------------UCGGGGGCCa-UUUGGU---------CGGU- -5' |
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| 23063 | 3' | -56.9 | NC_005178.1 | + | 12935 | 0.73 | 0.20541 |
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Target: 5'- gGACGUGGCgCgCCaGGUGcuGGCCGGCCu -3' miRNA: 3'- gCUGCAUCGgG-GG-CCAU--UUGGUCGGu -5' |
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| 23063 | 3' | -56.9 | NC_005178.1 | + | 23453 | 0.75 | 0.135634 |
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Target: 5'- gCGACGgccagGGCgCCCGGcgcgAAGCCGGCCc -3' miRNA: 3'- -GCUGCa----UCGgGGGCCa---UUUGGUCGGu -5' |
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| 23063 | 3' | -56.9 | NC_005178.1 | + | 33005 | 1.08 | 0.000496 |
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Target: 5'- uCGACGUAGCCCCCGGUAAACCAGCCAu -3' miRNA: 3'- -GCUGCAUCGGGGGCCAUUUGGUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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